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《Progress In Veterinary Medicine》 2001-04
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Using the Monoclonal Antibody-Based C-ELISA to Deteet S.enteritidis

ZHANG Yan hong 1,DU Yuan zhao 2,WU Yan gong 2,HUANG Su zheng 1,PENG Li ying 1 (1.Shanxi Province Epidemic Diseases Centre in Animals,Tai yuan,Shanxi, 030024,China;2.Animal Quarantine Research Institute of Agricultural Ministry,Qindao,Shandong  
We reported here establishment of a competitive enzyme linked immunosorbent assay(C ELISA) for detection of Salmonella enteritidis.A labelled minoclonal antibody(McAb)3 47 26,a specific for antigen 9 of S.enteritidis and S.enteritidis LPS in samples competed with S.enteritidis.LPS coated on microtitre plates for binding to the labeled McAb.The C ELISA was compared with the routine detective method.which detected 4.9×10CFU/g.and the C ELISA detected 5×10 4CFU of S.enteritidis/g of faeces,the C ELISA had sensitivity of 94.4% and specificity of 97.7%.Meanwhile,the importance and the selected requisition of the method were discussed.
【CateGory Index】: S852.61
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【Co-citations】
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