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《Progress In Veterinary Medicine》 2001-04
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Preparation of Monoclonal Antibody of Marek's Disease Virus (MDV) Glycoprotein B Expressed in E.Coli

HAN Ling xia 1,DING Jia bo 1,CHEN Lei 1 ,CUI Zhi zhong 2 (1.Vet. Med. Coll.,Yangzhou University,Yangzhou,225009, China;2.Anim. Sci. and Tech. Coll.,Shandong Agri. University, Taian, 271018, China)  
Glycoprotein B gene (gB) of virulent MDV GA strain expressed in E.coli was cut off from SDS PAGE ,then inoculated it to BALB/C mice directly to prepare monoclonal antibody. 1 strain of monoclonal hybridoma cells 7C8 was obtained by ELISA and Indirect Fluorescence Assay(IFA). Its ascites fluids titre of ELISA and IFA is 1: 212 and 1:800 respectively. The Mab can also identify CEF infected by MDV through dot ELISA.
【Fund】: 国家自然科学基金资助项目 (39870 0 0 8)
【CateGory Index】: S852.65
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【Citations】
Chinese Journal Full-text Database 3 Hits
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【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 JIANG Shi jin 1,CUI Zhi zhong 1,ZHANG Zhi 1,DING Jia bo 1,WANG Yu 1,YANG Han chun 2 (1.Animal Science and Technology College,Shandong Agricultural University,Shandong,Taian 271018,China; 2.Collage of Veterinary science,China Agricultural University,Beijing 100094,China);Cloning and Sequencing of pp24 Genes of Different Pathotype Reference Strains and Chinese Field Strains of Marek's Disease Virus Type Ⅰ(MDV-Ⅰ)[J];Chinese Journal of Virology;2004-01
2 Peng Daxin,Liu Xiufan,Wu Changxin,Wu Yantao,Wen Qiyi, Gao Song,Zhang Rukuan,Wu Xiaofeng,Xing Li,Zhu Aihua, Zhan Aijun (College of Animal husbandry Veterinary Science, Yangzhou University,Yangzhou 225009,China);PROTECTION STUDIES OF A RECOMBINANT FOWLPOX VIRUS VACCINE EXPRESSING GLYCOPROTEIN B GENE FROM CVI988/RISPENS STRAIN OF MAREK’S DISEASE VIRUS[J];Chinese Journal of Animal and Veterinary Sciences;2001-02
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5 HAN Ling-xia 1,DING Jia-bo 1,CHEN Lei 1,CUI Zhi-zhong 1,2* (1.College of Animal Husbandry and Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;2.College of Animal Science and Technology,Shandong Agricultural University,Taian 271018,China);Preparation of Monoclonal Antibody of Marek′s Disease Virus(MDV) Glycoprotein B Expressed in E.coli[J];Chinese Journal of Veterinary Science;2002-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
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【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Li Yi Cui Zhizhong Sui Dexin Lee L.F.(Jiangsu Agricultural College, Yangzhou China, 225001);EXPRESSION OF MAREKS DISEASE VIRUS gS GENE IN INSECT CELLS USING BACULOVIRUS AS A VECTOR[J];CHINESE JOURNAL OF VIROLOGY;1994-01
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