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《Progress in Veterinary Medicine》 2012-01
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Expression of E2 Protein of Classical Swine Fever Virus Shimen Strain Using Baculovrius Expression System

ZHOU Xiang-yang1,WAN Jing1,LIAO Xun2,ZHU Zhu-jun1, CHEN Xiao-wei1,LIU Fei-fen1,FANG Wei-huan2(1.Zhoushan Entry-Exit Inspection and Quarantine Bureau,Zhoushan,Zhejiang,316000,China; 2.Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine,Zhejiang University,Hangzhou,Zhejiang,310058,China)  
Expression of E2 protein of CSFV Shimen strain in baculovirus Bac-to-Bac expression system provided materials for research of the function of E2 protein and develope novel vaccine against CSFV.E2 gene of CSFV Shimen strain was subcloned into a baculovirus transfer vector pFastBacHTA to yield the recombinant transfer plasmid pFastBacHTA-E2.Recombinant bacmid was obtained after transforming the E.coli DH10Bac competent cells.Subsequently,the recombinant bacmid was transfected into Sf9 cells,then got the recombinant virus.The expressed recombinant protein was analyzed by Western blot and indirect immunofluorescence test.The results indicated that E2 protein was efficiently expressed,and had a specific reaction with monoclonal antibody 2B10 and 6×His monoclonal antibody.The E2 protein of CSFV Shimen strain was successfully expressed in Sf9 cells,and had a good antigenicity.
【Fund】: 浙江省自然科学基金(Y3110396)
【CateGory Index】: S852.651
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