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《Progress in Veterinary Medicine》 2012-01
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Sequence Analysis of E2 Gene of Classical Swine Fever Virus Strains Isolated from Some Areas of Beijing

FU Cai-xia,ZHENG Rui-feng,GUO Feng,JIN Xing-jun,WU Hui-ming, LI Jia,CHEN Li-ben,WANG Yu-tian (Beijing Laboratory Diagnosis Institute of Veterinary,Beijing,100101,China)  
In order to study genetic variation of classical swine fever virus(CSFV)strains in Beijing,the major antigen coding region sequences of E2 gene of seven CSFV strains isolated recently from some areas of Beijing were amplified by nested RT-PCR,cloned and sequenced.The amplified E2 gene sequences of these seven strains were analysed by DNAStar and compared with that of published CSFV strains.The results showed that the homologies of nucleotide and amino acid sequences of the seven strains were 96.3%-99.3%and 95.6%-100% respectively.The homologies of nucleotide and amino acid sequences of the seven strains to Shimen strain were 81.7%-83.5% and 82.4%-84.6% respectively.The homologies of nucleotide and amino acid sequences of the seven strains to HCLV strain were 80.6%-81.7% and 78.0%-80.2% respectively.The seven strains belonged to Group Ⅱ and variations of amino acids were observed at sites 705,713,729 and 734.This study revealed that the major antigen coding region sequences of E2 gene of CSFV strains isolated recently from some areas of Beijing were obviously different from that of Shimen and HCLV strains.
【Fund】: 生猪产业技术体系北京市创新团队建设项目(京农发[2009]44号)
【CateGory Index】: S852.651
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Wang Qin\ Wang Zaishi\ Zhao Yun\ Li Bo\ Qiu Huishen (National Control Institute of Veterinary Bioproducts and Pharmaceuticals, Beijing\ 100081);NUCLEOTIDE SEQUENCE ANALYSIS OF E2 MAJOR PROTECTIVE ANTIGEN ENCODING REGION OF 12 STRAINS OF HOG CHOLERA VIRUS(HCV)[J];ACTA MICROBIOLOGICA SINICA;2000-06
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 Tu Changchun Li Hongwei Jin Kuoshi Zhang Jingang Bai Anbin Liu Shiying Hu Rongliang Yin Zhen (Changchun University of Agriculture and Animal Sciences,Changchun,130012);SPECIFIC SEQUENCE AMPLIFICATION OF CHINESEHOG CHOLERA VIRUS STRAIN SHIMEN[J];CHINESE JOURNAL OF VIROLOGY;1994-01
2 Li Hongwei Tu Changchun Jin Kuoshi Wang Xingping Yin Zhen (Institute of Military Veterinary Medicine, Changchun Uniuversity of Aqricultural and Animal Sciences,Changchun 130062);CLONING AND SEQUENCE ANALYSIS OF E1 GENE OF TWO CHINESE HOG CHOLERA VIRUS STRAINS[J];CHINESE JOURNAL OF VIROLOGY;1998-03
3 FU Lie zhen, ZHU Yan, WANG Jia fu, ZHANG Peng wei, WANG Ning, ZHANG Chu yu (Institute of Virology, Wuhan University, Wuhan 430072,China);Nucleotide Sequence and Structure Analysis of E 2 Gene of Hog Cholera Lapinized Virus Chinese Strain[J];WUHAN UNIVERSITY JOURNAL(NATURAL SCIENCE EDITION);1999-04
4 Li Hongwei; Tu Changchun; Lu Zongji ;Jin Kuoshi; Yin Zhen (Changchun University of Agricultural and Animal Sciences, Changchun 130062);Sequence Analysis and Comparison of gp55 antigen encoding region of Hog Cholera Virus isolates of Changchun and Jilin[J];Virologica Sinica;1997-04
5 Han XueqingLiu XiangtaoZhao QizuLiu BohuaMa JunwuLi MinghuiE ChengjunLiu ZaixinLi JianqiangLi ZhongrunXie Qingge (Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu,730046);Analysis of Phylogenetic Relationship of Hog Cholera Viruses[J];CHINESE JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY;1999-06
6 YU Xinglong,LI Zousheng,TU Changchun, MA Zhenghai LI Hongwei,WU Jiangmin,YIN Zhen (The Mil.Vet.Inst Uni of Agri and Animal Sci,Changchun 130062,China)BZ Abstarct An indirect ELISA for the detection of antibodies against hog cholera virus(HCV)was d;Development of anIndirect ELISA for Detection of Antibodies against Hog Cholera Virus Employing Recombinan4 E2 expressedin E.coli[J];CHINESE JOURNAL OF PREVENTIVE VETERINARY MEDICINE;1999-03
7 LU Zong ji, LI Hong wei, TU Chang chun *, YU Xing long, WU Jian min, LI Yue hong, YIN Zhen * (The Military Veterinary Institute,Quartermaster University of PLA, Changchun 130062, China) [WT5HZ];Genetic Variations in Chinese Field Strains of Hog Cholera Virus[J];CHINESE JOURNAL OF VETERINARY SCIENCE;2000-04
8 Wang Xinping Tu Changchun  Li Hongwei  Jin Kuoshi  Xuan Hua Chang Guoquan  Sun Hongmei  Zhu Weizheng Fei Enge Yin Zhen  (Veterinary College, Uni of Agri and Anim Sci, Changchun 130062);Pigs Naturally Infected by Bovine Diarrhea Virus Present Signs Resembling Hog Cholera[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1996-04
9 Qiu Huishen, Lang Hongwu, Wang Zaishi The National Control Institute of Veterinary Bioproducts and Pharmaceuticals, Beijing 100081;Immunization with Attenuated Hog cholera Virus Chinese C Strain Protect Pigs Successfully Against Challenge with Virulent Field Strains[J];CHINESE JOURNAL OF VETERINARY DRUG;1997-03
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