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《Progress in Veterinary Medicine》 2012-02
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Study on Anti-PRRSV Effect of Lonicera japonica Extracts in Vitro

CHENG Feng,JIANG Yu-ting,YU Yun-fei,WANG Gui-ping,ZOU Kun,CHEN Jian-feng(Hubei Key Laboratory of Natural Products Research and Development,College of Chemistry and Life Science,China Three Gorges University,Yichang,Hubei,443002,China)  
The antiviral effect of Lonicera japonica on PRRSV in vitro was evaluated by observing cytopathic effect(CPE) on PRRSV infected Marc-145 cell,determining TCID50 and evaluating ORF7 mRNA expression level.The results showed that the 600 mL/L ethanol/water elution fractions possess the perfect protective effect on PRRSV infected cells and with the minimum protective concentration of 6.25 μg/mL.The 6.25 μg/mL 600 mL/L ethanol/water elution fractions decreased PRRSV titer from 105.8 TCID50 to 100.3 TCID50 and reduced the PRRSV ORF7 mRNA contents about 1 000 times.In summary,we disclosed 600 mL/L ethanol/water elution fractions of Lonicera japonica as a potential antiviral drug owing to its perfect anti-PRRSV effect.
【Fund】: 三峡大学博士启动基金(KJ2008B014)
【CateGory Index】: S853.73
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