Genetic Variation Analysis of F1L,B2L and VIR Genes of Orf Virus in Fujian Province

LIN Yu-sheng;JIANG Jin-xiu;JIANG Bin;YOU Wei;HU Qi-lin;Institute of Animal Husbandry & Veterinary Medicine,Fujian Academy of Agricultural Sciences;  

To investigate the variation in F1 L,B2Land VIR genes of Orf virus(ORFV),a total of 10 ORFV F1L,B2 Land VIR genes were cloned and sequenced from different farms in Fujian province during 2014-2015.The results showed that the nucleotide sequences of F1 Lgene shared 97.6%-100%similarities within them,shared 96.8%-99.7%similarities with domestic strains and shared 96.3%-97.1%similarities with NZ2 strain.Compared with NZ2 strain,there were 2amino acids deletion.The nucleotide sequence of B2 L gene shared 97.5%-99.9% similarities within them,shared 96.7%-99.5% similarities with domestic strains and shared 96.7%-97.7%similarities with NZ2 strain.The nucleotide sequence of VIR gene shared95.8%-99.5% similarities with them,shared 94.6%-99.6%similarities with domestic strains and shared94.6%-96.4% similarities with NZ2 strain.Phylogenetic analysis showed that F1 Lgene of the 10 ORFV strains had a closer relationship with the Fujian,Shanxi and Xinjiang strains;B2Lgene of the 10 ORFV strains had a closer relationship with the Xinjiang,Shanxi and Germany strains;VIR gene of 10 ORFV strains had a closer relationship with the Taiwan and Xinjiang strains.The results indicated that 10 ORFV F1L,B2 Land VIR genes have not a significant variation,but 10 ORFV F1L,B2 Land VIR genes have widespread heterogeneity between nucleotide sequences.

【Fund】： 福建省科技计划项目(2014R1102);; 福建省公益性科研专项(2014R1101002-8)
【CateGory Index】： S852.654

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