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《Progress in Veterinary Medicine》 2017-12
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Establishment of Duplex PCR for Detection of Infectious Bovine Rhinotracheitis Virus

XU Na;YANG Fan;LEI Yu;LI Ping-an;GUAN Ping-yuan;College of Veterinary Medicine,Inner Mongolia Agricultural University;Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture;  
To establish a sensitive detection protocol of infectious bovine rhinotracheitis virus(IBRV),the quantitative real-time PCR was developed with 2 pair of primers targeting the conserved region of IBRV gE and gB genes.The result showed that the established assay possessed high specificity for amplification of IBRV,but no amplifiction of bovine mycoplasma,bovine parainfluenza virus type 3,bovine viral diarrhea virus,Pasteurella,Burcella.This research established the PCR detection method that was of the advantages of rapid,sensitive,accurate and could be used for IBRV detection.
【Fund】: 国家科技支撑计划项目(2015BA107B02)子课题(2015BA107B02-07)
【CateGory Index】: S852.653
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