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《Progress in Veterinary Medicine》 2017-12
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Preparation and Identification of Hybridoma Cell Lines Secreting Antibodies against Goat IFN-gamma

ZHANG Hong-jie;SANG Feng-feng;LIU A-Hua;LI Yan-yan;WANG Yi;CHEN De-kun;College of Veterinary Medicine,Northwest A&F University;  
In order to prepare hybridoma cells secreting monoclonal antibodies against goat IFN-γ,Balb/c mice were immunized with purified prokaryotic recombiant goat IFN-γ(rIFN-γ)and the splenocytes were isolated and fused with SP2/0 myeloma to generate hybridoma cells.Then the supernatant was collected to identify the monoclonal antibodies recognizing goat IFN-γspecifically.In the present study,an I-ELISA method was established to detect antibodies against goat IFN-γwhen the antigen was coated with goat lymphocytes.Then the classes and types of monoclonal antibodies against goat IFN-γwere characterized by quick test strip.The results suggested that a strain of hybridoma cell named 3 Cwhich showed high specificity was prepared successfully,and the monoclonal antibody was identified as IgG class and kappa type.
【Fund】: 基金项目:陕北白绒山羊主要疫病防控关键技术研究与示范(2015KTTSNY04-04)
【CateGory Index】: S852.4
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