Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Progress in Veterinary Medicine》 2017-12
Add to Favorite Get Latest Update

Genetic Variations of ORF5 Genes of Porcine Reproductive and Respiratory Syndrome Isolated in Guangdong Regions from 2016 to 2017

YU Lin-yang;DONG Jian-guo;ZHANG Le-yi;LIU Yan-ling;LIANG Peng-shuai;WANG Lei;SONG Chang-xu;College of Animal Science,South China Agriculture University;College of Animal Husbandry and Veterinary,Xinyang College of Agriculture and Forestry;  
To investigate the genetic variations of ORF5 genes of porcine reproductive and respiratory syndrome virus(PRRSV)isolated in Guangdong regions,the lung samples of pigs suspected to be PRRSV positive from pig farms in Guangdong during 2016 to 2017 were collected and the ORF5 of the PRRSV in these samples were amplified by RT-PCR.The ORF5 gene sequences and deduced amino acid sequences were furtherly analyzed.Twelve complete ORF5 gene sequences were amplified,and phylogenetic analysis results showed that 10 isolates had close genetic relationship with the highly pathogenicitic PRRSV(HPPRRSV)strains and they belonged to the same cluster.Other 2 isolates had close genetic relationship with the newly isolated strain QYYZ and they belonged to the same cluster.The amino acids alignment results indicated that the epitope of GP5 had extensive amino acid mutations.These results indicated that the region's PRRSV strains have experienced extensive evolation.These results not only revealed that the genetic evolution characteristics of PRRSV ORF5 gene in Guangdong,but also provided a scientific basis for furtherly controlling the PRRS.
【Fund】: 国家支撑计划项目(2015BAD12B02-5);; 广州市科技计划项目(201508020062);; 现代农业产业共性技术创新团队项目(2016LM2150)
【CateGory Index】: S852.651
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved