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《Progress in Veterinary Medicine》 2017-12
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Development of a Multiplex Polymerase Chain Reaction Assay for Detection of Common Pathogens in Cow Mastitis

DIAO Qiao-hong;BIAN Guo-zhi;WANG Juan;Luo Ai-jian;ZHANG Ying;GAO Xiao-yi;JIA Kun;SUN ling-shuang;YUAN Jian-feng;College of Veterinary Medicine,South China Agricultural University;Guangdong Haid Institute of Animal Husbandry & Veterinary;Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases;Guangdong Haid Group Co.,Limited;  
To establish a rapid assay for Streptococcus agalactiae,Streptococcus dysgalactiae,Streptococcus uberis and Staphylococcus aureus detection,in this study,four pairs of specific primers were designed according to the sequences published in GenBank,by the sipgene of Streptococcus agalactiae,ispof Streptococcus dysgalactiae,pauA of Streptococcus uberis and nuc of Staphylococcus aureus.Development of the PCR assay for detection of these bacteria.The result showed that the assay had the advantages of high sensitivity and good specificity,the sensitivity of S.agalactiae,S.dysgalactiae,S.dysgalactiae,S.uberis and S.aureus were 105 CFU/mL,104 CFU/mL,105 CFU/mL and 105 CFU/mL,respectively.The performance of the multiplex PCR was evaluated by examining 460 clinical mastitis milk samples.The results showed that the multiplex PCR can be used for detection these four bacteria in milk samples.
【Fund】: 广州市科技计划项目(201510010250);; 广东省科技计划项目(2014A020208052 2015B020203005 2015A020224039);; 广东省兽医临床重大疾病综合防控重点实验室项目(2013A061401013);; 广东省现代农业科技创新联盟建设项目(2016LM2150);; 广州市珠江科技新星专项(201610010073)
【CateGory Index】: S852.61
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