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《Progress in Veterinary Medicine》 2017-12
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Sequence Analysis of Meq Gene of Three Marek's Disease Virus Strains in Henan Province

ZHANG Tian-wei;ZHANG Jian-jun;HE Hong-xuan;Wanxi Technical School;Sheqi Teacher Training School;Institute of Zoology,Chinese Academy of Sciences;  
To investigate genetic variation of Marek's disease virus(MDV)in Henan province,one pair of primers for amplifying Meq gene of MDV were designed according to nucleotide sequence in GenBank,and three isolates of MDV were detected from infected chickens by PCR.Targeted fragments were cloned and sequenced,then compared with different types of reference MDV strains published in GenBank.The results showed that Meq gene from the three MDV isolates consisted of 1 020 bp.Compared with reference strains published in GenBank,the sequences of Meq gene in different isolates were relatively conserved and the homologies of nucleotide and amino acid sequences of the isolates were more than 98.6% and more than 96.5% .The isolates displayed amino acid site mutations at 9 positions.Phylogenetic analysis showed that the isolates and strains could be separated into three groups.The Henan1-3 strains,ZC2014(KP144356.1),YLO40920(DQ174459.1),HNXZ106(HF546099.1),and vv+MDV648 A(AY362725.1)were clustered into one group,vMDV GA(AF147806.1),vvMDV RBIB(AY243332.1)clustered into the second group,and mMDV CVI988(AF493555.1)and Chinese vaccine 814 strains(AF493551)were clustered into the third group.
【CateGory Index】: S852.65
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