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《Progress in Veterinary Medicine》 2017-12
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Principle and Application of Insulated Isothermal PCR

WANG Juan;BIAN Guo-zhi;WANG Gui-ping;YUAN Jian-feng;Guangdong Haid Institute of animal Husbandry & Veterinary;College of Veterinary Medicine South China Agricultural University;  
Because the heating mechanism used in convention PCR based on thermal conductivity still require a delicate digital controller to rapidly heat and cool the sample,in recent years,agrowing number of studies have focused on developing a fast and portable PCR platform for nucleic acid testing.Natural convection,another mode of heat transfer,promotes spontaneous and repeated liquid circulation to provide working temperatures for denaturation,annealing and extension.Insulated isothermal PCR(iiPCR),a rapid nucleic acid detection method,based on Rayleigh-Be′nard convection principle,amplification is carried in an iiPCR device in which a special polycarbonate capillary tube(R-tubeTM)is heated isothermally by a copper ring attached to tis bottom and shielded by a thermal baffle around its upper half,spontaneous fluid convection in a R-tube is driven by temperature gradients for PCR reaction.The results show directly on LED display.Compared with the traditional PCR;iiPCR assay shows its advantages in the rapid detection of clinical nucleic acid,and has begun to use in the field of clinical diagnosis of various pathogens for its low cost,simple and easy operation,high specificity,high sensibility and high amplification efficiency.Here we proceeded a review for insulated isothermal PCR covering its introduction,research progress and application prospect.
【Fund】: 广东省科技计划项目(2014A020208052);; 广州市科技计划项目(201510010250)
【CateGory Index】: S854.4
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