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《Progress in Veterinary Medicine》 2018-05
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Effects of Compound Chinese Herbal Medicinal Polysaccharides on IL-1β,TNF-α and IL-8 mRNA Expressions in Lymphocytes of Different MHC B-LβⅡ Genotype Chickens

MA Zhao;LUO Yan;LIU Gang;YANG Li;LIU Xiao-ting;ZHU Xiao-qing;GU Xin-li;College of Animal Science and Technology,Shihezi University;  
To investigate the effects of compound Chinese herbal medicinal polysaccharides on the expressions of IL-1β,IL-8,TNF-α mRNA in lymphocytes of chickens with different MHC B LβII genotypes,PCR-SSCP method was used to group 500 layers of Jinghong 1 according to different MHC B-LβII genotypes.The peripheral blood lymphocytes of different MHC B-LβII chickens were collected and the compound Chinese herbal medicinal polysaccharides were added to the final concentrations of 100,75,50,0 g/mL respectively,and cultured for 24 hours.The cells were collected.The expressions of IL-1β,TNF-α,IL-8 mRNA in lymphocytes of chickens with different MHC B-LβII genotypes were detected by real-time PCR.The results showed that compound Chinese herbal medicinal polysaccharides at different does could improve mRNA expressions of IL-1β,TNF-α,IL-8 genes in chickens with different MHC B-LβII genotypes(P0.05);When the dosage of compound Chinese herbal medicinal polysaccharides was 50μg/mL,the expressions of mRNA of IL-1β,TNF-α,IL-8 genes were the highest in the AA BC genotype chickens,and the highest expressions of mRNA levels were found in the IL-1β,TNF-α,IL-8 genes in the chickens when the dosage of compound Chinese herbal medicinal polysaccharides was 100μg/mL.Compound Chinese herbal medicinal polysaccharides can promote the expressions of mRNA of IL-1β,TNF-α,IL-8 genes in different MHC B-LβII genotypes of chickens,and the most suitable dosage of compound Chinese herbal medicinal polysaccharides for immune regulation of lymphocytes in different MHC B-LβII genotype chickens is slightly different.
【Fund】: 国家自然科学基金项目(31460673)
【CateGory Index】: S853.74
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