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《Progress in Veterinary Medicine》 2018-06
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Detection of Trichuris suis and Its ITS Sequence Analysis

GUO Nan-nan;ZHANG Fan-fan;TANG Yu-xin;ZHANG Min;WU Jia-bing;WU Qiong;YE Yu;SONG De-ping;College of Animal Science and Technology,Jiangxi Agricultural University;Key Laboratory for Animal Health of Jiangxi Province;  
Trichuris suis are the common intestinal parasites in human and animals.In November 2015,the finishing pigs in a farm in Jiangxi province had severe diarrhea with blood in feces,leading to die of some pigs.Anatomical diagnosis found the intestine was filled with linear-like worms.Through the observation of the onset symptoms,morphology of the worms and eggs,it was initially identified as infection of Trichuris suis.Then a 1,345 bp fragment of the internal transcribed spacer(ITS)sequence was amplified and sequenced.Blast analysis indicated that ITS sequence had 99.8%identity with that of Trichuris suis.Phylogenetic analysis showed the relationship with other strains was far,but the nucleotide homology was still more than 93.5%.Most importantly,it had highest identities with a Trichuris suis strain(AM993005)from Yiyang of Hunan Province,China.Therefore,this study provided a technical reserve and theoretical guidance for the establishment of the molecular biology diagnostic method of Trichuris suis.
【Fund】: 国家自然科学基金项目(31372457);; 江西省教育厅科技计划项目(GJJ160399)
【CateGory Index】: S858.28
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