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《Progress in Veterinary Medicine》 2018-06
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Cloning and Prokaryotic Expression of VP3 Gene of Novel Duck Parvovirus

JING Mei;WANG Jian-chang;CUI Yuan;LI Xiao-xuan;CHEN Ping;WANG Jin-feng;HOU Shao-hua;SUN Ji-guo;YUAN Wan-zhe;College of Veterinary Medicine,Agriculture University of Hebei;Inspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau;Beijing Institute of Animal Husbandry and Veterinary Medicine,Chinese Academy of Agricultural Sciences;Hebei Engineering and Technology Research Center of Veterinary Biotechnology;  
In this research,VP3 gene of novel duck parvovirus(NDPV)was amplified by PCR and cloned into the prokaryotic expression vector pQE1.The recombinant plasmid pQE1-VP3 was optimized in induced expression conditions.The expressed products were analyzed by SDS-PAGE.Results showed that VP3 protein of NDPV was expressed successfully in prokaryotic cells.The protein could obtain maximum expression at 25℃ and 6 hour when the concentration of IPTG was 1.0 mmol/L.The molecular weight of the expressed protein was about 60 ku,mainly existed in the form of insoluble inclusion body.After purification,the protein concentration and purity were respectively 5.165 mg/mL and 97%.In conclusion,VP3 protein of NDPV was efficiently expressed in prokaryotic cells and which laid the foundation for further study on NDPV detection methods and new vaccine.
【Fund】: 河北省高校百名优秀创新人才支持计划(SLRC2017039);; 国家重点研发计划项目(2017YFD0500806)
【CateGory Index】: S852.65
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