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《Progress in Veterinary Medicine》 2018-06
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Pathogenic Characterization of Streptococcus suis Serotype 2 in Infected Pig Groups across China-Myanmar Border

ZHENG Yu-fang;LU Qiong-fen;LI Xiao-hong;ZHANG Rong-ping;CHEN Pei-fu;College of Veterinary Medicine,Yunnan Agricultural University;  
In mid-October 2016,there was an outbreak of a contagious disease in several pig farms in the China-Myanmar border,which was characterized by dyspnea,joint swelling,discharge of yellow stool in partial piglets,neurological symptoms at the late stage and sepsis before death.In order to investigate its etiology in detail,the whole blood,lung tissue,pustule and pus were collected from the diseased pigs,then cultured by streaking on blood agar,followed by apreliminary identification of the resulting hemolytic colony using Gram staining,biochemical test,drug susceptibility test and agglutination test based on sera from chronic cases;apair of universal primers for bacterial 16S rRNA genes were subsequently used to amplify the isolates corresponding DNA fragment by PCR,and the PCR products were subjected to TA cloning as well as sequence analysis.Consequently,the hemolytic strain was spherical and positive for Gram staining.The biochemistry tests of glucose,sucrose,lactose,maltose,salicylic acid and raffinose were positive,but tests of sorbitol,esculoside,hippurate,mannitol,65 g/L NaCl,inositol and catalase were negative.It had a high sensitivity to cefotaxime,cefoperazone,enrofloxacin and sulfamethoxazole,a moderate sensitivity to penicillin,amoxicillin,gentamicin and ciprofloxacin,and a strong resistance to tetracycline,doxycycline,kanamycin,neomycin,florfenicol,amikacin,lincomycin and other drugs.Agglutination test was positive with a seral antibody titer of 256.PCR yielded a single 1 500 bp band that had the highest sequence identity up to 99% with Streptococcus suis type 2 sequence type 1(ST1),thus the isolated strain was determined to be S.suis type 2.Since these piglets were once inoculated with S.suis type 2 inactivated vaccine,our findings here demonstrated that this cross-border infectious disease in the local herds was caused by multi-drug resistant S.suis type 2,indicating the importance of monitoring changes of the bacterial antigenicity,pathogenicity and drug resistance and strict control of cross-border flow of pigs.
【Fund】: 云南省高校人兽共患病重点实验室(A3007954);; 云南省现代农业生猪产业技术体系建设(云财农[2009]171号)
【CateGory Index】: S858.28
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