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《Progress in Veterinary Medicine》 2018-06
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Effects of Reduced Glutathione on Testis of Mice with Fluorosis

ZHANG Xiao-qing;LI Su-juan;CAO Guo-di;SUN Zi-long;College of Shuozhou Vocational and Technology;College of Animal Science and Technology,Shanxi Agricultural University;  
In order to study the effect of different dosages of reduced glutathione on the testis of mice with fluorosis,50 ICR male mice which were 3 weeks old were randomly divided into five groups:control group and four fluoride group.The control group and four fluoride groups were orally administrated with 0 and100 mg/L NaF for 30 d.Selecting 3 groups from 100 mg/L NaF group were divided into 100 mg/L NaF+200 mg/kg GSH group,100 mg/L NaF+400 mg/kg GSH group and 100 mg/L NaF+800 mg/kg GSH group,and the 3 groups of mice were given daily with doses of 200 mg/kg GSH,400 mg/kg GSH and 800 mg/kg GSH,respectively.At the same time,the 5 groups of mice were free drinking fluoridated water for30 days.After feeding time,the level of ROS,MDA and GSH,the activity of GPx,GSTs and GR in the testis were detected.And paraffin sections were prepared and analyzed by HE staining.The results showed that compared with the control group,the contents of ROS and MDA in the 100 mg/L NaF group increased significantly,and the activities of GSTs and GR decreased significantly.Compared with the 100 mg/L NaF group,the activity of GR increased significantly in the GSH group and 100 mg/L NaF+800 mg/kg GSH group.Compared with the control group,100 mg/L NaF seminiferous tubule structure sparse,cell layer thickness,spermatogenic cells arranged in disorder,some spermatogenic cells shed,lumen radius becomes large and the central lumen sperm significantly decreased;with the increase of GSH concentration,the mice in 100 mg/L NaF+800 mg/kg GSH group recover maximum.After adding GSH,the enzyme activity in glutathione antioxidant system of mice with fluorosis increased,the testis morphology recovered to a certain extent,having certain effect on the testis.
【Fund】: 国家自然科学基金项目(31201965);; 山西省高等学校优秀青年学术带头人支持计划项目
【CateGory Index】: R599.1
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