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《Progress in Veterinary Medicine》 2018-06
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Fermentation Optimization of Bacillus subtilis Expressing rBovMtb8.4

LI Hai-tao;ZHANG Bin;LIU Yan-huan;ZHU Yan-zhu;GU Chen-chen;YAN Xi-jun;MIAO Li-guang;Institute of Special Wild Economic Animal and Plants,CAAS;Key Laboratory of Special Animal Epidemic Disease,Ministry of Agriculture;Veterinary Biological Products Supply Station,Shijiazhuang;  
In order to establish preparation method of Bacillus subtilis fermentation expression of Mycobacterium bovis Mtb8.4 antigen,the constructed plasmid containing pNC-HisE-Mtb8.4 expression vectors was used to express in the system of secretory expression of Bacillus subtilis.The expression yield was optimized in shake flask level by the single factor influence test.The results showed that the optimum conditions for each factor were 2 SY medium,inoculation concentration of 6%and 18 hoptimum seed stage,shaking culture 48 hat 33℃,and the expression of target protein could reach 750 mg/L.The establishment of the expression condition laid the foundation for the enlargement of the later production process.
【CateGory Index】: TQ920.6
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