Electron Microscopy on the Freeze-fracture Replica of the Human Epidermis on the Low Impedence Lines along Channels
Wang Zhongtao et al(Department of Histology and Embryology)Hebei Medical College, Shijiazuang 050017)
Electron microscopy on the epidermis of the skin on the low impedence lines of 10 human legs were studied by the method of freeze—fracture replica. It was revealed that the basal cells possess many processus; there are many endocytotic pores on their cell membrane; and the intercellular space between them is about 1 μm in width. In the cytoplasm of these cells, microfilaments and mitochondria are distributed randomly, Golgi complex and smooth surfaced endoplasmic reticulum are occasionally revealed. The prickle cells have more cytoplasm within which membrane—coating granules (MCG) are ocurred. The granular cells have more MCG and exocytotic pores than those of the prickle cells. The horny cells are scale in shape, its cell membrane thinckens enormously, on its fractured faces, except the desmosome area, there is no intramembraneous particle and exocytotic pore. The position, size and structure of desmosomes on various cells are different. In the prickle cell layer, gap junction and square array are revealed, while in the granular cell layer, tight junction is seen. Filamentous structure abutted on the horny cell membrane is also demonstrated.