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《Journal of Chinese Electron Microscopy Society》 2008-05
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Effect of different preparation condition on SpA-molecularly imprinted polymer microspheres

XUE Xiu-heng,PAN Jian,XIE Hui-ming,ZHANG Shuang(Engineering Research Center of Bio-Process,Ministry of Education,He fei University of Technology,Hefei Anhui 230009,China)  
SpA-Molecularly imprinted polymer microspheres were prepared by inverse suspension polymerization using Staphylococcus aureus protein A as template and acrylamide as functional monomer.The polymer under different conditions was evaluated by using ESEM to select the best technology.The adsorption property of SpA molecularly imprinted polymer was detected.The results show that the best condition are that EC is 0.20 g/100 mL Tol,The ratio between toluene and water is 10∶3 crosslinking degree is 10%,initiator adding manner is dropping,the reaction temperature is 50℃.The adsorbance of SpA molecularly imprinted polymer microspheres prepared by inverse suspension polymerization is 6.956×10-3 μmol/g.
【Key Words】： Staphylococcus aureus protein A molecularly imprinted polymer microsphere inverse suspension
【Fund】： 安徽省“十一五”重大科技项目(06003015B)
【CateGory Index】： O631
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