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Effect of drought stress on ultrastructure of flag leaves in wheat chromosome substitution lines

BAI Zhi-ying~1,LI Cun-dong~ 2 ,QU Ping~1(1.College of Life Science,2. College of Agronomy, Agricultural University of Hebei,Baoding Hebei 071000,China)  
The differences of cell ultra-structure of flags leaves in two wheat substitution lines and parents under drought stress were investigated. The result showed that the ultra-structure of the chloroplasts and nucleus as well as mitochondria of 2D and 5D substitution lines along with parents were alike under normal irrigation. The cell ultra-structure of flags leaves of 2D substitution line and Synthetic 6x with strong drought resistant were less affected than 5D substitution line and Chinese Spring with weak drought resistant under drought stress. The chloroplast with a few starch grains and plentiful grana lamellae of 2D substitution line and Synthetic 6x became a little swelling, whether the nucleus and mitochondria showed no obvious change. The chloroplasts of 5D substitution line and Chinese Spring became more round, thylakoid of chloroplast became swollen, matrix lamella became obscure, the number of lipid body balls increased, the starch grains decreased, a few chloroplast began to disintegrate; Part of envelope of mitochondria with indistinct cristae began to breakage, the nucleus also showed no obvious change. The changes of flag leaves were negative correlation with wheat drought-resistant.
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