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Comparative study on the structure and aggregation behavior of ophiopogon japonicus polysaccharides extracted by two methods

WANG Xiao-mei1,SUN Run-guang1,ZHANG Jing2,HAO Chang-chun1,ZHANG Li-ni1(1.College of Physics and Information Technology,2.College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi’an Shaanxi 710062,China)  
In this paper,different extraction methods including hot water and ultrasound were used to extract polysaccharides in ophiopogon japonicus.The functional group distribution and surface morphology of polysaccharides extracted by the two methods were studied by FT-IR spectroscopy,atomic force microscopy(AFM) and scanning electron microscopy(SEM).The results showed that the difference of functional group between ophiopogon japonicus polysaccharides extracted by hot water(WPOJ-DS) and ultrasound(UPOJ-DS) was obvious.β-glucopyranoside was detected in WPOJ-DS using infrared spectroscopy,but not in UPOJ-DS.The results of AFM showed that the dispersion of UPOJ-DS was significantly higher than that of WPOJ-DS.Single molecule chain and helical structure were observed in UPOJ-DS by AFM,indicating that intermolecular and intramolecular hydrogen bonds of polysaccharides were affected by ultrasound.The results of SEM showed that less of bigger schistose structures were observed in UPOJ-DS compared to WPOJ-DS,and a lot of rod-shaped and small globular aggregates were observed in UPOJ-DS,indicating that supersonic treatment created degradation on ophiopogon japonicus polysaccharides.Therefore,ultrasonic extraction has an effect on molecular structure and aggregation behavior of ophiopogon japonicus polysaccharides.
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