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《Journal of Chinese Electron Microscopy Society》 2013-01
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Comparative study on the structure and aggregation behavior of ophiopogon japonicus polysaccharides extracted by two methods

WANG Xiao-mei1,SUN Run-guang1,ZHANG Jing2,HAO Chang-chun1,ZHANG Li-ni1(1.College of Physics and Information Technology,2.College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi’an Shaanxi 710062,China)  
In this paper,different extraction methods including hot water and ultrasound were used to extract polysaccharides in ophiopogon japonicus.The functional group distribution and surface morphology of polysaccharides extracted by the two methods were studied by FT-IR spectroscopy,atomic force microscopy(AFM) and scanning electron microscopy(SEM).The results showed that the difference of functional group between ophiopogon japonicus polysaccharides extracted by hot water(WPOJ-DS) and ultrasound(UPOJ-DS) was obvious.β-glucopyranoside was detected in WPOJ-DS using infrared spectroscopy,but not in UPOJ-DS.The results of AFM showed that the dispersion of UPOJ-DS was significantly higher than that of WPOJ-DS.Single molecule chain and helical structure were observed in UPOJ-DS by AFM,indicating that intermolecular and intramolecular hydrogen bonds of polysaccharides were affected by ultrasound.The results of SEM showed that less of bigger schistose structures were observed in UPOJ-DS compared to WPOJ-DS,and a lot of rod-shaped and small globular aggregates were observed in UPOJ-DS,indicating that supersonic treatment created degradation on ophiopogon japonicus polysaccharides.Therefore,ultrasonic extraction has an effect on molecular structure and aggregation behavior of ophiopogon japonicus polysaccharides.
【Fund】: 国家自然科学基金资助项目(No.10874108);; 教育部科学技术研究重点资助项目(No.SJ08A16)
【CateGory Index】: S567.232
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 CAI Lin tao\ LI Ping *\ LU Zu hong (National Laboratory of Molecular and Biomolecular Electronics,Southeast University,Nanjing 210096,China.) (* Department of Pharmacognosy,China Pharmaceutical University,Nanjing 210009,China.);Observation of the structure morphology of cordyceps polysaccharide by atomic force microscope[J];JOURNAL OF CHINESE ELECTRON MICROSCOPY SOCIETY;1999-01
2 WANG Derun, YU Xianchao(The Central Lab. of Nankai University, Tianjin)ZHAO Dajian, CAO Wuxiong and FAN Tingyi(Department of Biology of Nankai University, Tianjin);STUDY OF THE APPEARANCE OF XANTHAN MOLECULES[J];Acta Polymerica Sinica;1990-01
3 SUN, Run-Guang*,a ZHANG, Jingb (a Laboratory of Biophysics and Biomedical Technology, College of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062) (a Department of Food Engineering, Shaanxi Normal University, Xi'an 710062);A Study of Helical Structure of Glycyrrhiza Polysaccharides by Atomic Force Microscope[J];Acta Chimica Sinica;2006-24
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 FAN Jian-ping 2,YANG Yong-li 1*,ZHANG Ji 1, GUO Shou-jun 1,YAO Jian 1,WANG Xing-dong 3(1 College of Life Science,Northwest Normal University,Lanzhou 730070,China;2 Biology Department,Qinghai Normal University,Xining 810008,China;3 No.12 Middle Sc;The synergy studies of sophora bean gum with xanthan gum[J];Acta Botanica Boreali-occidentalia Sinica;2002-02
2 ZHU Huai-jiang,ZHAO Chang-qing,LUO Jian-hui,WEI Bao-he,YANG Jing-bo,ZHU De-sheng (Research Institute of Petroleum Exploration & Development, Petrol-China,Beijing 100083,China);Study on the microstructure of polymer molecule hydrate[J];Journal of Chinese Electron Microscopy Society;2005-03
3 DAI Ling~1,SHEN Ye-shou~1,ZHUANG Yong-long~2,ZHAO Zhi-ping~1,LIAO Hong-mei~1(1 School of Life Science,Anhui University,Anhui Key Laboratory of Eco-engineering & Bio-technique,Hefei Anhui 230039;2 Modern Experiment Center,Anhui University,Hefei Anhui 230039,China);TEM observation of Polysaccharide from Root of Paeonia suffruticosa Andr[J];Journal of Chinese Electron Microscopy Society;2006-05
4 XU Ping-ping1,SUN Run-guang 1 ,ZHANG Jing2,LIU Na-n1,ZHANG Jing3,LI Dai1,WANG Xiao-mei1(1. College of Physics and Information Technology,2. Department of Food Engineering,Shaanxi Normal University,Xi'an Shanxi 710062;3. Xi'an University of Petroleum,Xi'an Shanxi 710061,China);Study of structure of polysaccharides from Ligustrum Lucidum Ait by atomic force microscopy[J];Journal of Chinese Electron Microscopy Society;2009-02
5 QIN Li-hong1,CAO Jian-bo1,YI Wei-song 2 (1. The Public Labratory of Electron Microscope,Huazhong Agricultural University,Wuhan Hubei 430070;2. College of Science Huazhong Agricultural University,Wuhan Hubei 430070,China);A new method for scanning electron microscope samples of polysaccharides distilled from green tea and the observation of ultrafine structure of them with atomic force microscope[J];Journal of Chinese Electron Microscopy Society;2009-02
6 LI Dai,ZHANG Jing~*,SUN Run-guang,GUO Shu-qin(Laboratory of Biophysics and Biomedical Technology,College of Physics and Information Technology,ShanxiNormal University,Xi'an Shanxi 710062,China);Thermal properties and aggregation behaviors of acidic bulpeurum polysaccharide WBP_(011)[J];Journal of Chinese Electron Microscopy Society;2009-03
7 WANG Derun and YU Xianchao(Central Laboratory, Nankai University, Tianjin)ZHAO Dajian(Department of Biology, Nankai University, Tianjin);STUDY ON CONFORMATIONS OF XANTHAN MOLECULES IN AQUEOUS SOLUTION[J];Acta Polymerica Sinica;1990-03
8 CUI Meng zhong 1, LI Zhu yun 2, XU Shi ai 1 (1.Key Laboratory of Polymer Science & Engineering, Yantai University, Yantai, 264005, China; 2.Department of Applied Chemistry, Yantai University, Yantai 264005, China);Progress of Properties、Appliances & Functionalize of Biopolymer Xanthan Gum[J];Polymer Bulletin;2003-03
9 WANG Xiu-yan, DONG Li-song * (State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China);Advances in Study on the Structure of Polysaccharide by Atomic Force Microscopy[J];Polymer Bulletin;2004-01
10 YANG Jie~1,CHU Ping~1,XIONG Yu-hui~1,WANG Rui~1,TANG Yu-ping~2,DUAN Jin-ao~2 (1.State Key Laboratory of Pharmaceutical Biotechnology,College of Life Sciences,Nanjing University,Nanjing 210093,China; 2.Nanjing University of Traditional Chinese Medicine,Nanjing 210046,China);Computer-aided drug design using in the modernization of traditional Chinese medicine[J];World Clinical Drugs;2009-10
China Proceedings of conference Full-text Database 2 Hits
1 ;羊栖菜多糖(SFPS-B2)的原子力显微镜观察[A];[C];2008
2 Zhang Jing~*,Liu Hong, (College of Food Engineering and Nutritional Science,Shaan Xi Normal University,Xi,an 710062,China);Biological Activity,Determination of Molecular Structure and Extraction Technology of Pumpkin Polysaccharide[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 SHI Lei 1, WANG Yong 1,SHI Yong 2, LI Juan 2, LIU Zhi qiang 1, LIU Shu ying 1 (1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022,China;2. School of Public Health, Jilin University);Observation of Polysaccharide from Ornithogalum caudatum Ait. under Atomic Force Microscope[J];Journal of Norman Bethune University of Medical Science;2003-01
2 NI De-Jiang, CHEN Yu-Qiong, XIE Bi-Jun *, ZHANG Yun, ZHOU Ji-Rong (College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China);Spectrum, Morphological and Thermal Characteristics of OTPS 2-1 in Polysaccharides from Oolong Tea[J];Chemical Research In Chinese Universities;2004-12
3 SUN, Run-Guang ZHANG, Jing QI, Hao (College of Physics and Information Technology, Department of Food Engineering, College of Life Science, Shaanxi Normal University, Xi′an 710062);Study of Stability and Structure of Lipid Bilayer Membrane by Atomic Force Microscope[J];Acta Chimica Sinica;2002-05
4 ZHANG, Jinga SUN, Run-Guang*,b (a Department of Food Engineering, Shaanxi Normal University, Xi'an 710062) (b College of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062);Effect of Saccharide and Salts on the Stability of Super-Molecular Ordered Structure of Simulated Biomembrane[J];Acta Chimica Sinica;2006-19
5 MA Xiu li, BAI Yu bai, SUN Yun xiu, LIU Ju zheng (Department of Chemistry, Jilin University, Changchun, 130023) LIU Zhong ying (Norman Bethune University of Medical Sciences, Changchun, 130021);Observation of Polysaccharide from Panax Quinquefolium L. PPQ-d by Atomic Force Microscopy[J];ACTA SCIENTIARIUM NATURALIUM UNIVERSITATIS JILINENSIS;2000-01
6 ZHANG Jing,LIU Ping,CAI Li(Department of Food and Engineering, Shaanxi Normal University, Xi′an 710062, Shaanxi, China);Extraction and determination of water-soluble polysaccharide of Glycyrrhiza[J];Journal of Shaanxi Normal University(Natural Science Edition);2005-02
7 Gao Xiaorong, Liu Peixun (Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China);Advances in study on structure analysis of polysaccharides[J];Tianjin Pharmacy;2003-06
8 Sun Run-Guang 1) Qi Hao 2) Zhang Jing 3) 1)(College of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062,China) 2)(College of Life Science, Shaanxi Normal University,Xi'an 710062,China) 3)(Department of;Structures and properties of liposome by atomic force microscope[J];Acta Physica Sinica;2002-06
9 Wu Shi Ying Zhang Yi Lei Xiao Ling Hu Jun Ai Xiao Bai Li Min Qian (Shanghai Institute of Nuclear Research, Chinese Academy of Sciences,Shanghai 201800, China);The catenary-like nano-pattern of individual DNA molecules constructed by liquid flow manipulation[J];Acta Physica Sinica;2002-08
10 Zhao Dajian;Yiang Jianhua;Yu Xianchao;Wang Derun Department of Biology, Nankai University, Tianjin Central Laboratory, Nankai University, Tianjin;MOLECULAR WEIGHT OF XANTHAN POLYSACCHARIDE[J];Chinese Journal of Applied Chemistry;1989-05
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