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《Journal of Chinese Electron Microscopy Society》 2013-06
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Improved sample preparation method for scanning electron microscopic observation of apoptotic cells

ZHAO De-zhang;LUO Zi-guo;YANG Jie-shun;Institute of Life Science,Chongqing Medical University;  
Apoptosis samples prepared by conventional sample preparation method for scanning electron microscopic observation demonstrate lower detection rate of apoptosis under microscope,and cellular exfoliation and deformation are easily found,which does not meet the observation requirements. In this study,prostate cancer PC-3 cells were treated by 200 μmol / L dihydroartemisinin for 48 h to induced cellular apoptosis. Improved sample preparation method was employed and the ultrastructure of apoptotic cells was observed. The results revealed that the positive detection rates of apoptotic cells and apoptotic bodies were significantly increased and the cells attached to the cover slips tightly and distributed evenly,and the ultrastructural images of apoptosis obtained from scanning electron microscope were more intuitive and visual than that from transmission electron microscope.
【Fund】: 重庆市科委资助项目(No.cstc2012ggyyjs10017 超微病理快速诊断与远程实时互动平台构建)
【CateGory Index】: Q2-33
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