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《Journal of Chinese Electron Microscopy Society》 2014-01
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Electron microscope observation of Salmonella Pullorum transmembrane tunnel mediated by the bacteriophage lysis protein E

GUO Rong-xian;GENG Shi-zhong;JIAO Hong-mei;JIAO Xin-an;PAN Zhi-ming;CHEN Yi-fang;AN Shu-min;Jiangsu Key Laboratory of Zoonoses,Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University;School of Medicine,Yangzhou University;Testing Center of Yangzhou University;  
Bacteriophage lysis protein E can lead to a fusion of the inner and outer membranes to form a specific transmembrane tunnel in Salmonella cytoderm. In this study,scanning electron microscopy( SEM) and transmission electron microscopy( TEM) were used to observe the characteristic of the transmembrane tunnel. Bacterial ghosts were prepared by inducing expression of the bacteriophage lysis gene E in Salmonella Pullorum S06004 harboring plasmid pBBR1MCS2-E. The pre-treatment of samples were observed by SEM and TEM. After induced in 42 ℃,the vast majority of Salmonella Pullorum recombinants could form a transmembrane tunnel at the polar sites of bacteria with a diameter varying in the range of 200 ~ 400 nm. Bacterial ghosts show the loss of cytoplasmic material and maintain the integrity of the outer membrane structure. The cell surface wrinkled obviously. This research provides an efficient morphological evidence for Salmonella ghosts as vaccine candidate,which is mediated by the bacteriophage lysis protein E.
【Fund】: 863计划(No.2012AA101601-6);; 国家自然科学基金资助项目(No.31230070)
【CateGory Index】: S858.31
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