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《Journal of Chinese Electron Microscopy Society》 2014-01
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The application of Hexamethyl disilazane( HMDS)in sample preparation of plant tissues for scanning electron microscopy( SEM)

LI Jian-xia;ZHAO Liang-cheng;WANG Jian-zhong;XIA Xiao-fei;College of Nature Conservation,Beijing Forestry University;College of Biological Sciences and Biotechnology,Beijing Forestry University;Beijing Museum of Nature History;  
Samples of plant tissues for SEM are usually dried by methods of critical point drying and freeze-drying. However,there are limitations with these methods,such as complex operations,specialized equipment requirement and so on. In this paper,the samples were dried with Hexamethyl disilazane( HMDS). The effect was compared with those using the freeze-drying method. The results showed that samples in young tissues,such as the flowering stigma and ovary,could be dried well by both the HMDS method and the freeze-drying method and the effects of them on sample drying are similar. By contrast,the effects of HMDS on drying of mature ovule tissues were worse than that of the freeze-drying method.
【Fund】: 北京市科学技术研究院青年骨干计划(2012-017)
【CateGory Index】: TQ028.8
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