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《Journal of Chinese Electron Microscopy Society》 2014-01
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The application of Hexamethyl disilazane( HMDS)in sample preparation of plant tissues for scanning electron microscopy( SEM)

LI Jian-xia;ZHAO Liang-cheng;WANG Jian-zhong;XIA Xiao-fei;College of Nature Conservation,Beijing Forestry University;College of Biological Sciences and Biotechnology,Beijing Forestry University;Beijing Museum of Nature History;  
Samples of plant tissues for SEM are usually dried by methods of critical point drying and freeze-drying. However,there are limitations with these methods,such as complex operations,specialized equipment requirement and so on. In this paper,the samples were dried with Hexamethyl disilazane( HMDS). The effect was compared with those using the freeze-drying method. The results showed that samples in young tissues,such as the flowering stigma and ovary,could be dried well by both the HMDS method and the freeze-drying method and the effects of them on sample drying are similar. By contrast,the effects of HMDS on drying of mature ovule tissues were worse than that of the freeze-drying method.
【Fund】: 北京市科学技术研究院青年骨干计划(2012-017)
【CateGory Index】: TQ028.8
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Chinese Journal Full-text Database 2 Hits
1 XIAO Yuan,LIU Wei,WANG Yan,ZUO Yan-xia,HU Rui,LI Ting-ting,CUI Zong-bin(Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China);Drying Methods of Biological Sample Preparation for Scanning Electron Microscope[J];Research and Exploration in Laboratory;2013-05
2 Jianxia Li;Chulan Zhang;Xiaofei Xia;Liangcheng Zhao;College of Biological Sciences and Biotechnology,Beijing Forestry University;Beijing Museum of Nature History;;Cryo-sectioning Conditions and Histochemistry Comparison with Paraffin Sectioning[J];Chinese Bulletin of Botany;2013-06
Chinese Journal Full-text Database 10 Hits
1 ZHOU Xian-han et al(School of Biotechnology and Food Engineering of Hefei University of Technology,Engineering Reserch Center of Bio-process Ministry of Education,Hefei,Anhui 230009);Research on the Optimum of the Technical Condition in the Bacillus-killing with Supercritical CO_2[J];Journal of Anhui Agricultural Sciences;2010-17
2 YE Hui-ling et alDepartment of Internal Medicine, The Medical College of Shantou University, Guangdong. P.R. China;AN ELECTRON-MICROSCOPIC STUDY ON NON-HODGKIN'S LYMPHOMA(NHL)[J];Chinese Journal of Cancer;1986-04
3 CAO Jin PAN Qi-chao(Department of Anticancer Drug Research; Cancer Institute) ( Sun Yan-Sen University of Medical Sciences, Guangzhou 510060);THE DAMAGES OF THE TOTAL ALKALOID OF HARMALA ON THE ULTRASTRUCTURE OF RETICULUM-CELL SARCOMA L2 CELLS IN MICE[J];Chinese Journal of Cancer;1993-03
4 Hong Tao Xia Shimao Zhou Jingyi Liao Huaxin Fu Jianlin(Institute of Virology, China National Center for Preventive Medicine);INCLUSION BODY-AN IMPORTANT MORPHOLOGICAL MARKER OF EHF VIRUS[J];Chinese Journal of Virology;1985-01
5 Li Cheng Gu Shouli Wang Jike Liu Shushen Jiang Saode Hao Guiyu Zhang Tan(Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin);A MODEL STRUCTURE OF EQUINE INFECTIOUS ANEMIA VIRUS[J];Chinese Journal of Virology;1985-03
6 Du Nianxing Xu Weiyan Liu Shengjiang (Division of Veterinary Microbiology, Nanjing Agricultural University, Nanjing);A PRELIMINARY REPORT ON THE IDENTIFNTIFICATION OF A NEW VIRUS --RABBIT HEMORRHAGIC DISEASE VIRUS[J];Chinese Journal of Virology;1986-02
7 Li cheng Gu Shoulin Wang Jike Jiang SaodeHao Guiyu Zhang Tan( Harbin Veterinary Reseach Institute, Chinese Academy of Agricultural Sciences );SOME ABNORMAL VIRUS PARTICLES OBSERVED UNDER ELECTRONMICROSCOPE[J];Chinese Journal of Virology;1987-01
8 Wang Changan Yang Rongjian Liang XiaoguangHung Tao Institute of Virology, Chinese Academy of Preventive MedicineJiangxi Institute of Medical SciencesFuxin Municicipal Health Station;OBSERVATION ON THE DURATION OF ADULT DIARRHEA ROTAVIRUS EXCRETION FROM PATIENTS' FECES[J];Chinese Journal of Virology;1987-04
9 Chen Feng Jiang Jiyuan Lin Jihung Shen Jiangping Department of Biotechnology, Chtna Pharmacy UniversityInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences;VIRUS-LIKE PARTICLES OBSERVED IN A LOCAL BUFFALO'S DISEASE[J];Chinese Journal of Virology;1988-03
10 You Zhiyong Wang Yiming Zhao Zhiguo He YingXu Puting Zhao Zijiang Cheng YubengCheng Wenzhou Zhang Hongfi Institute of Virology, Chinese Academy of Preventive Medicine, Beijing Hygiene and Epidemic Prevention Station of Hainan Province,Haikou Hygiene and Epidemic Prevention Station, Zhelimumong, the Inner Mongolia Autonomous Region;ISOLATION AND IDENTIFICATION OF TWO STRAINS OF ORBIVIRUS IN HAINAN PROVINCE[J];Chinese Journal of Virology;1990-03
China Proceedings of conference Full-text Database 2 Hits
1 ;新型组织工程化骨修复骨缺损的实验研究[A];[C];2010
2 YU Wan-wen~1 CAO Fu-liang~1 ZHU Zun-ling~2 ZHOU Chun-li~2 LIU Xin-liang~1 (1 College of Forest Resources and Environment,2 College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037);Research on Leaf Color Difference of Golden-leaf Ginkgo's Offsprings[A];[C];2013
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 QIAN Tian-le et al(Cooperative Laboratory of Experimental Center of Life Science College and Techcom Corp.,Nanjing Agricultural University,Nanjing,Jiangsu 210095);Improvement of Bathing Method of Microorganism Samples for SEM and Comparison of Their Fixing and Dryness Methods[J];Journal of Anhui Agricultural Sciences;2009-23
2 CAO Jun-mai,BEI Zhan-lin,JIANG Yong.WEI Jie,XU Xin (College of Biological Science and Engineering,North University of Ethnics,Yinchuan.Ningxia 750021);Flower Bud of Lycium barbarum.L Scanning Electron Microscopy Sample Prepartion Methods Discussde[J];Northern Horticulture;2010-03
3 Zhang Xiancai1,Zhang Lei2(1.Department of Anatomy Histology and Embryology,Xingtai Medical College,Xingtai,Hebei 054000,China;2.Department of Histology and Embryology of Hebei Medical University,Shijiazhuang 050017,China);Observation of small intestinal mucosa of diabetic mice by scanning electron microscope[J];Chongqing Medicine;2011-28
4 Liu Jianfeng, Yan Xiufeng(Northeast Forestry University, Harbin 150040 P. R. China); Cheng Yunqing, Li Xia(Jilin Normal University);Application of Cryo-sectioning Technique to Cytology Studies of Rhodiola sachalinensis A. Bor[J];Journal of Northeast Forestry University;2006-04
5 WAN Yi zhen,HE Pu chao (College of Horticulture,Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);The technic parameters of slicing vitis berries by freezing-microtome[J];Acta Botanica Boreali-occidentalia Sinica;2001-02
6 LI Lei~1,LIU Qi-zhi~(1*),WANG Yu-zhu~2,JIA Jun-zhen~3,SUN Hao-yuan~2,LIU Hai-hong~3(1.Entomology and Nematology Laboratory,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094,China;2.Pomology and Forestry Institute,Beijing Agriculture and Forestry Science Academy,Beijing 100093,China;3.College of Biological Sciences,Electronic Microscope Laboratory,China Agricultural University,Beijing 100094,China);Technique comparison of SEM sample preparation on nematodes[J];Journal of Chinese Electron Microscopy Society;2008-03
7 WANG Li,YAN Jing~*,ZHANG Lian-wei,LEI Jian-zhang(Department of Electron Microscopy,Hebei Medical University,Shijiazhuang Hebei 050017,China);Scanning electron microscopic observation of goblet cell[J];Journal of Chinese Electron Microscopy Society;2008-04
8 KONG Xiang-lin~1,LUO Rong~2,LIU Xian-lin~1,SU Xiao-qing~(2*)(1.The Key Laboratory of EM,Guiyang Medical College,2.Department of Biology,Guiyang Medical College,Guiyang Guizhou 550004 China);An observation on hyphae and asexual reproductive stages of Pythium guiyangense Su,a mosquito-killing fungus,with scanning electron microscope[J];Journal of Chinese Electron Microscopy Society;2008-04
9 GUO Su zhi, JI Qing e (Test Centre of Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Drying methods of insect specimens for scanning electron microscopy[J];Journal of Fujian Agricultural University(Natural Science);2001-02
10 GUO Su-zhi~1, ZHANG Shu-ling~2, CHEN Yu-fen~2, ZHANG Shao-sheng~2 (1.Test Centre;2.College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Sampling method of Ditylenchus destructor specimens for scanning electron microscopy[J];;2005-01
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