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Application of laser scanning confocal microscopy(LSCM)in primary renal tubular epithelial cells adhered to uropathogenic Escherichia coli

WU Kun-yi;SONG Yun;YUAN Guo-lian;ZHANG Ting;WANG Jia-xing;LI Ke;Core Research Laboratory,The Second Affiliated Hospital,School of Medicine,Xi'an Jiaotong University;  
Objective: To investigate the mechanism of bacterial adhesion to renal tubular epithelial cells( PTEC),we observed the colonization of pathogenic Escherichia coli and mannose in PTEC. Methods: Under the stimulation of anaphylatoxin C5 a and C5 aR antagonist( PMX53),the expression of mannose on PTEC and adhesion of PTEC to bacteria was observed by laser scanning confocal microscopy( LSCM). Results: The mannose expression was mainly distributed around the nucleus in normal PTEC; after bacterial stimulation,the expression of mannose was significantly increased,and the co-localization of bacteria with cell surface mannosyl residues on PTEC was also observed; after stimulated with C5 a or bacteria,the adhesion of bacteria to PTEC and the expression of mannose were up-regulated significantly,and co-localization of bacteria with mannose was enhanced notably; however,under the stimulation of PMX53 and bacteria, the adhesion of PTEC to bacteria and mannose expression were significantly decreased.Conclusion: C5 a changes the expression of mannose on the cell surface,which maybe affect the adhesion of bacteria to PTEC.
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