Study of Identifying the Varieties of Populus by the means of RAPD
Wang Jianya, Huang faxin, Eiji HAYASHI, Kaichino KAWAMURA (Hubei Province forest Tree Breeding Center,Wuhan 430079)
The best reaction composition have been studied in this paper. It is that 10xbuffer 1. 50μl,dNTP 0. 30μl,MgCl2 1. 60μl,h20 10. 57μl,primer 1. 00 μl,TaqDNA 0. 03μl.Template DNA 1. 00μl. The results show htat: 32 polymorphic markers have been found when 60 primers were used to identify the 13 varieties of Populus,and 13 arbitrary markers can identify them completely.
【CateGory Index】： S792.11