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《Journal of Fudan University》 2001-01
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GNA Confers Resistance to Rice Brown Planthopper in Transgenic Rice Pure Lines by Both Bioassay and Feeding Tests

TANG Ke-xuan1,WAN Bing-liang2,SUN Xiao-fen1, QI Hua-xing2,LU Xing-gui2 (1.State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China; 2. Hybrid Rice Research Center, Hubei Academy of Agricultural S  
Two transgenic rice pure lines, generated by particle bombardment, were challenged by the first-instar nymphs of rice brown planthopper (Nilaparvata lugens,BPH) biotype I using the standard BPH bioassay and feeding test protocol essentially the same as described by Rao et al. (1998). Both lines contained and expressed the selectable marker hygromycin phosphotransferase gene(hpt), the reporter β-glucuronidase gene(gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna). Insect bioassay and feeding test results showed that both lines had significant deterrent effect on BPH by decreasing BPH survival and overall fecundity, retarding BPH development and declining BPH feeding.
【Fund】: SupportedbyChinaNationalHigh Tech“86 3”Program (GrantNo .10 1 0 1 0 1 0 1) ;SupportedbyChinaMinistryofEducation ;Supportedby
【CateGory Index】: Q943.2
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