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《Journal of Fudan University》 2004-02
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Complete Nucleotide Sequence Analysis of Two Corynebacterial Plasmids pXZ10142 and pXZ10145 Containing a Transposon-like Sequence Tn45

REN Zhao-rui~(1), SHI Shao-lin~(1), JIANG Xiao-bin~(1), YAN Wei-yao~(1), LEI Cheng-xiang~(2), LIU Ming-qiu~(1), ZHENG Zhao-xin~(1) (1.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China; 2.Naval Medical Research Institute, Shanghai 200433, China)  
Two plasmids, pXZ10145.1 and pXZ10142, isolated from one strain of Corynebacterium glutamicum 1014 were analysed. Comparison between the pXZ10145.1 and pXZ10142 sequences showed that the plasmid pXZ10142 originated in the excision from the plasmid pXZ10145.1. The sequence of pXZ10145.1 revealed 6 open reading frames (ORFs). ORF1 encoding 247 amino acids encompassed the replication protein, which was proved by deletion analysis. A transposon-like structure Tn45 was implied from the DNA sequence of pXZ10145.1. It contained a CMR gene, a TNP gene, and two inverted repeat sequences IR1a and IR1b, which are located at the junction between (pXZ10145.1) and pXZ10142. The direct repeat sequences are ATCTAG flanking both inverted repeat sequences of IR1a and IR1b.
【Key Words】: Corynebacterium glutamicum plasmid pXZ10145.1 pXZ10142 transposon Tn45
【CateGory Index】: TQ925
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Chinese Journal Full-text Database 1 Hits
1 LI Kai~ 1,2 ,ZHAO Zhi~2,ZHANG Ying-zi~2,WANG Yu~2,DING Jiu-yuan~ 2 ( ~1 Institute of Microbiology,Chinese Academy of Sciences, Beijing 100080,China)( ~2 Graduate School of Chinese Academy of Sciences, Beijing 100039,China);Construction of Corynebacterium glutamicum/E.coli shuttle promoter-probe vector[J];Acta Microbiologica Sinica;2007-02
Chinese Journal Full-text Database 2 Hits
1 Zheng Zhaoxin Ma Chuping Yan Weiyao He Peifu Mao Yixiang Sun Wei(Institute of Gmetics, Fudan University, Shanghai) Lei Zaozu Zhu Ping Wu Jufen(Institute of Industrial Microbiology, Shanghai);RESTRICTION ENDONUCLEASE MAP OF PLASMID pXZ10145 IN CORYNEBACTERIUM GLUTAMICUM AND THE CONSTRUCTION OF COMPOSITE PLASMID[J];Chinese Journal of Biotechnology;1987-03
2 Lei Chengxiang Fan Changsheng Huang Weida Shen Shuiyuan Yan Weiyao Zheng Zhaoxin (National Laboratory of Genetics Engineering, Fudan university, Shanghai 200433);Cloning and Expressing of Escherichia coli Gene pheA in Brevibacterium flavum[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1996-S1
Chinese Journal Full-text Database 7 Hits
1 LI Ji-xin,ZHANG Chao(Xinjiang Academic of Agriculture and Reclamation Science,Shihezi 832000;Southern Yangtze University,Key Laboratory of Food Science and Safety,Wuxi 214036,China);Progress of L-Phenylalanine Manufacture and Application[J];Amino Acids & Biotic Resources;2006-02
2 LU Jun, TANG Jiong, ZHANG Wen bo, YAN Wei yao, ZHENG Zhao xin (State Key Laboratory of Genetic Engineering, School of Life Sciences);Construction of a Expression Vector pJL23 in Corynebacteria[J];Journal of Fudan University;2000-03
3 WANG Cheng-hua,LIU Bao-sheng,HUA Wei,ZHANG Qiang(The Key Labratory for Biochemical Engineering of Shandong Province,Jinan 250014,China);Cloning and Expressing of dapA Gene in Corynebacteria[J];Shandong Chemical Industry;2005-03
4 JIANG Pei Hong, LIU Ai Min, GE Hai Peng, ZHANG Yuan Ying, FAN Chang Sheng and HUANG Wei Da ( School of Life Science, Fudan University, Shanghai 200433, China );Cloning and Expression of aro G Gene of E.coli and Its Co expression with phe A and tyr B Genes[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1998-06
5 ZENG Xiao\|Bing 1\ FAN Chang\|Sheng 1\ JIANG Pei\|Hong 2 CHEN Yong\|Qing 1\ HUANG Wei\|Da 2 ( 1Department of Microbiology and Microbial Biotechnology, 2Department of Biochemistry,Fudan University,Shanghai 200433);Co-expression of Heterologous Genes pheA,aroG and tyrB for Biosynthesis Pathway of L-phenylalanine[J];CHINESE JOURNAL OF BIOTECHNOLOGY;2000-06
6 Fan Changsheng Zeng Xiaobing Chai Yunrong ( Departmentof Microbiology Fudan University Shanghai China 200433)Jiang Peihong Huang Weida ( Departmentof Biochemistry Fudan University Shanghai China 200433);EXPRESSION OF GENES aroG AND pheA IN PHENYLALANINE BIOSYNTHESIS[J];ACTA MICROBIOLOGICA SINICA;1999-05
7 LEI Chengxiang FAN Changsheng DUAN Fenghai HUANG Weida SHEN Shuiyuan YAN Weiyao and ZHENG Zhaoxin (State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China);Construction of shuttle expression vector and breeding of genetic engineered strains for L-phenylalanine[J];自然科学进展(英文版);1996-02
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 YANG Yu,HUANG Zhi-ying,DIAO Meng-xue,QIU Guan-zhou(School of Material Proceeding and Bioengineering,Central South University,Changsha 410083,China);Construction of Promoter Probe Vector for Acidithiobacillus ferrooxidans[J];Biotechnology;2008-05
2 Chunhua Zhang1,2 , Zhi Zhao1 , Yingzi Zhang1, Yu Wang1, Jiuyuan Ding1 (1Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China) (2Graduate school of the Chinese Academy of Sciences, Beijing 100049, China);Cloning, expression and sequence analysis of DSⅠgene in Corynebacterium pekinense AS1.299 and PD-67[J];Acta Microbiologica Sinica;2008-11
3 Guiming Liu1,2,Zhi Zhao1,Yingzi Zhang1,Yu Wang1,Jiuyuan Ding1(1 Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)(2 Graduate School of Chinese Academy of Sciences,Beijing 100049,China);Cloning and analysis of promoter-active fragments from Corynebacterium glutamicum 10147[J];Acta Microbiologica Sinica;2009-07
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Qian Hong Fan Wei Wu Junlin Zheng Zhaoxin (Institute of Genetics, Fudan university, Shanghai, 200433);Plasmids Transfer from Eschenchia coli to Corynebacterium by Conjugation[J];Chinese Journal of Biotechnology;1994-01
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