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《Journal of Fudan University(Natural Science)》 2012-06
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A Preliminary Study of Key Regulated Enzymes Forglucosamine Metabolism in Escherichia coli BL21(DE3)

TU Yu-peng,HE Jing-hua,LE Ke-yi,YAN Wei-yao(Department of Microbiology and Microbiological Engineering,School of Life Sciences, Fudan University,Shanghai 200433,China)  
As a class of widely used healthcare products in the world,glucosamine(GlcN) provides good regeneration of human joint cartilage and palliation the pain or disability of osteoarthritis(OA).For the purposes to create a new method of GlcN production based on metabolic engineering,operon manXYZ(mannose-specific PTS enzymes) and operon nagBACD-nagE(include acetyl-glucosamine-specific transporter,deacetylase,deaminase and its regulatory proteins) of Escherichia coli BL21(DE3) were deleted using λRed homology recombination system.A new expression vector pETG was constructed in order to highly express the GlmS protein.Compared to the wild type Escherichia coli BL21(DE3),the engineered strain failed to use glucosamine or acetyl-glucosamine as carbon source.By the modified Morgan-Elson method,an 8.63 U/mg activity of glmS protein was identified after purification by Ni-NTA affinity chromatography.These suggest that the result bacterial has initial characteristics of the ideal GlcN production strain.
【CateGory Index】: Q786
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【References】
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1 The Chinese Western Normal University of Chemistry and Chemical Engineering;;The chemical structure,properties and chemical synthesis of Glucosamine[J];同行;2016-15
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【Co-references】
Chinese Journal Full-text Database 5 Hits
1 TU Yu-peng,HE Jing-hua,LE Ke-yi,YAN Wei-yao(Department of Microbiology and Microbiological Engineering,School of Life Sciences, Fudan University,Shanghai 200433,China);A Preliminary Study of Key Regulated Enzymes Forglucosamine Metabolism in Escherichia coli BL21(DE3)[J];复旦学报(自然科学版);2012-06
2 Xin Chen1,2,Long Liu1,2,Jianghua Li1,2,Jie Liu3,Guocheng Du1,2,and Jian Chen1,41 Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,Jiangsu,China2 School of Biotechnology,Jiangnan University,Wuxi 214122,Jiangsu,China3 Aland(Jiangsu) Nutraceutical Co.,Ltd,Jingjiang 214500,Jiangsu,China4 National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,Jiangsu,China;Influence of nagE and manX knockout with Red homologous recombination on the microbial production of glucosamine by Escherichia coli[J];生物工程学报;2012-03
3 LI Yun-Man 1 ,TANG Ming-Yue 1 ,XIA Wen 2 ,LI Te 1 1 Department of Physiology,China Pharmaceutical Univer sity,Nanjing 210009; 2 Department of Blood,Jiangsu Province Hospital of Traditional Chine se Medicine,Nanjing 210009,China;Antitumor Effect of D-Glucosamine Hydrochloride in Mice[J];中国天然药物;2003-04
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5 Xia Wenshui Liu Xiazhong(School of Food Science & Technology,Wuxi University of Light Industry,Wuxi,214036);Stability and Preparation of D Glucosamine Hydrochloride[J];无锡轻工大学学报;1997-04
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1 SHANG Haitao;Anhui BBCA Group Co.,Ltd.;;Study on Fermentation Conditions of Glucosamine[J];甘肃农业科技;2017-01
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