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《Journal of Fudan University(Natural Science)》 2018-01
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Improvement of Specific Primer Design on 16S rDNA and Its Application on Skin Microbes Detection of Trachidermus fasciatus

XU Penghao;HE Enming;WANG Yanlei;CAI Feng;ZHANG Liang;DOU Tonghai;WANG Jinqiu;ZHOU Yan;Department of Microbiology and Microbial Engineering,School of Life Sciences,Fudan University;Shanghai-MOST Key Laboratory of Health and Disease Genomics,Chinese National Human Genome Center at Shanghai;State Key Laboratory of Genetic Engineering,Fudan University;  
The specificity that primer pairs binding with template is one of main factor determining the success of PCR reaction.And general demands of primer specificity occur when dealing with microbe identification based on16 SrDNA and various detection.While high coverage of target genus is needed to amplify most species,the primer pairs is supposed to be special enough to avoid wrong amplification of other genus sequence,which refers to specificity among genus.This experiment programs a suited primer design software aimed at this problem.First,the analysis of all known bacteria 16 SrDNA are taken.And primer pairs are screened aimed at target genus.Moreover,an evaluation of primer efficiency is taken to gain efficient primers.A flora analysis of Trachidermus fasciatus'skin microbes were done in order to check the validity of primer design.The result shows great coverage and specificity of primer with a relatively high efficiency.The primer pairs designed are suited for flora analysis.
【Fund】: 上海市自然科学基金项目(16ZR1423900);; 国家高技术研究发展计划课题(2012AA020409)
【CateGory Index】: Q78
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