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《Journal of Fudan University(Natural Science)》 2018-02
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Aspergillus fumigatus COFILIN Interacts with Human GOSR1 and Inhibits HEK293T Cell Proliferation

SHAO Junhua;SHI Chao;WANG Yaqian;HUO Keke;School of Life Sciences,Fudan University;State Key Laboratory of Genetic Engineering,Fudan University;  
Aspergillus fumigatus COFILIN is an actin depolymerizing factor in eukaryote,which can promote cytoskeletal dynamics.COFILIN protein not only participate in the process of infecting host cells,but also helped host cells escaping from immune system defenses.In order to explore the functions of COFILIN in host cells,researchers firstly found COFILIN could interact with GOSR1(Golgi SNAP receptor complex member 1)through yeast two-hybrid screening.Then by using GST pull-down and Co-IP assay,researchers confirmed the specific interaction between the two proteins in vitro and in vivo,and identified the interaction domain by constructing deletion mutants.By analyzing the expression of GOSR1 in HEK293 Tcells,researchers concluded that exogenous COFILIN could suppress the expression of GOSR1 in mRNA and protein level.Besides,it could inhibits cell proliferation,promote cell apoptosis,and causes G2/M block.
【Fund】: 国家重点基础研究发展计划(2013CB531603)
【CateGory Index】: R519
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