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《Journal of Fudan University(Natural Science)》 2018-02
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Effect of Jab1 Gene RNAi on Proliferation and Migration of Breast Cancer MCF-7 Cells

ZHU Genfeng;YU Tonglu;CAI Dongliang;YE Xiaojuan;MIN Taishan;CHEN Haoming;LU Daru;School of Life Sciences,Fudan University;MOE Key Laboratory of Contemporary Anthropology,Fudan University;  
In breast cancer cell line MCF-7,successfully established a lentivirus mediated RNAi of Jab1 gene expression.CCK8 cell proliferation assay and cell scratch experiments confirmed that knock down of Jab1 gene expression makes the proliferation and migration of MCF-7 cells were significantly decreased.Cell cycle analysis showed that knock down of Jab1 gene expression can increase the proportion of sub G1 phase in the MCF-7 cells and induce apoptosis.in addition,the RT-PCR and Western blot confirmed that Jab1 can regulate the expression of TBHS1,Add the methyltransferase inhibitor 5-Aza-dC can inhibit the expression of Jab1 interference mediated down-regulation of THBS1 expression,suggesting that Jab1 can regulate the expression of THBS1 by epigenetic signaling pathways.This research will be helpful to reveal the molecular mechanism of Jab1-THBS1 signaling pathway in Breast cancer cells.
【Fund】: 国家自然科学基金(81071739 81272385)
【CateGory Index】: R737.9
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