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《Chinese Journal of Luminescence》 2017-09
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Mechanism of Interaction Between Ceftazidime and Trypsin by Spectroscopic and Molecular Docking Methods

WANG Jin-ju;LIU Bao-sheng;BIAN Gang;DUAN Shao-tong;LI Tong-tong;Key Laboratory of Analytical Science and Technology of Hebei Province,College of Chemistry & Environmental Science,Hebei University;  
The binding of ceftazidime( CFD) with trypsin( TRP) was investigated by spectroscopic and molecular docking methods under different temperature conditions( 298,303 and 310 K). The results demonstrate that the interaction between CFD and TRP is taking place via static quenching with 1∶ 1 binding ratio. The fluorescence data were treated by using the double logarithmic equation,and the binding constants Kaof the interaction of CFD-TRP systems and the number of binding sites n were obtained. The thermodynamic parameters of CFD-TRP systems under different temperatures were obtained by the thermodynamic equation. The experimental data show that the interactions between them are mainly hydrophobic interaction and hydrogen bonding interaction,which is consistent with the molecular docking results.
【Fund】: 国家自然科学基金(21375032);; 河北省重点基础研究项目(10967126D)资助~~
【CateGory Index】: O657.3;R96
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