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《Environmental Science & Technology》 2008-11
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Degradability of Poly-γ-glutamic Acid in Environment

CHEN Xiong1, CHEN Shou-wen2, YU Zi-niu2(1.School of Bioengineering, Hubei University of Technology, Wuhan 430068, China; 2.School of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)  
Degradability of poly-γ-glutamic acid (γ-PGA) in water environment and soil environment was studied by measuring the variety of poly -γ -glutamic acid and glutamic acid with high performance liquid chromatography. Results showed that the degradation rate of γ-PGA was small in water environment,with only 1.6% γ-PGA degraded and content of glutamic acid 120mg/kg after 10d while the degradation rate of γ-PGA was great in soil,with only 7.6% γ-PGA reserved and content of glutamic acid up to 3.2mg/g after 20d. Half-life of γ-PGA in sterilized soil was 3.7times that of in unsterilized soil,which indicated that the degradation of γ-PGA in the soil was mainly due to microbial action. B.subtilis CCTCC202048 producingγ-PGA,isolated from soil,can degrade γ-PGA and γ-PGA depolymerase gene was identified by PCR. The study would lay a foundation for the application of γ-PGA in agriculture.
【CateGory Index】: X172
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