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《Environmental Science & Technology》 2009-02
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Optimum Conditions of Improved MTT Assay for Estimating in Vitro Acute Cytotoxicity in Different Fish Cells

WANG Yu-fei,WU Hong-juan,WEN Chen,LIU Guang-hong (School of Environmental Science and Engineering,Huazhong University of Science and Technology,Wuhan 430074,China)  
Tests were done to determine the optimum conditions of improved MTT assay for estimating in vitro acute cytotoxicity of fish cell through Channel Catfish Ovary(CCO)cell,Ctenpharyngoden Idellus Kindy(CIK)cell and Epithelioma Papulosum Cyprinid(EPC)cell. Results showed that under the optimum conditions of wavelength 570nm and incubation time 4h,MTT concentration was 1mg/mL,with inoculation cell number depended on characteristics of cell and increased with growth speed of the cell.
【Fund】: 农业部948课题资助(2005Z37);; 湖北省科技攻关课题(2005AA401C41)
【CateGory Index】: X503.225
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【Citations】
Chinese Journal Full-text Database 2 Hits
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