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《Environmental Science & Technology》 2009-02
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Color Correction in Luminescent Bacteria Toxicity Test Utilizing Light Emitting Diode for Measuring Toxicity of Colored Water Samples

QU Jing-yan, WANG Guang-he, LU Xiang-yue (Department of Environment Science and Technology,East China Normal University,Shanghai 200062,China)  
When colored water sample was measured with luminescent bacteria toxicity test,the color of sample will affect the detection for luminescence. A light emitting diode (LED)installation was used to correct the depression of luminescence which was caused by sample color. There was difference between bacteria and LED luminescence by detecting edible colorant solution,then the coordinate rotation converting method was used to rectify the difference,which was authenticated using simulated water sample made by edible colorant and phenol. Finally,the toxicity of activity blank KN-B solution was detected by the method,and co-concentric double-deck test tubes were used to check the reliability of rectification. Results showed that the relative errors between sample toxicity and real toxicity were less than 5%,which indicated the method was effective. EC50 of KN-B was 213.9mg/L,which was similar to the result detected by double-deck test tubes. The method was found available to reject the luminescence depression caused by sample color.
【Fund】: 国家高技术研究发展“863”计划专项基金(2003AA601020)
【CateGory Index】: X832
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