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《Environmental Science & Technology》 2010-01
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Isolation and Identification of Microcystins

YU Ting,XIE Ping,DAI Ming,LIANG Gao-dao(Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China)  
Microcystins are a kind of hepatotoxic cyanotoxin.Extraction of intracellular Microcystin variants is required to characterize and quantify all microcystins present in a sample.The method was established to isolate and purify Microcystins from natural cyanobacterial bloom dominated by Microcystis aeruginosa in Dianchi Lake,Yunnan Province,by the main steps of extraction with 75% aqueous methanol,5% acetic acid solution through semi-prep-LC and ODS column with purity over 90% .The study was to develop an appropriate analytical procedure,based on solid phase extraction and liquid chromatography electrospray mass spectrometry for the determination of trace amounts of MCs in water samples.The developed method was applied to analyze the Microcystin-polluted surface water sampled from East Lake,Wuhan.
【Fund】: 国家自然科学基金项目(30530170)
【CateGory Index】: X52
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