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《Environmental Science & Technology》 2010-01
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Fungal Laccase as Catalyst for Polymerization of Lignin from Paper Mill Effluent

WU Xiang-bo1,XIE Yi-min1,FENG Xiao-jing1,2(1.Key Joint Laboratory of Paper Science and Technology of Ministry of Education,Shandong Institute of Light Industry,Jinan 250353,China;2.Shandong Academy of Environmental Science,Jinan 250013,China)  
To confirm the catalytic polymerization role of laccase on lignin from the effluent after secondary sedimentation tank of paper mill,the effect of fungal laccase from Coriolus versicolor on lignin polymerization was investigated.Laccase and ABTS mediator were added to effluent sample under aerobic condition.The change of lignin content was determined by UV spectrometer and increase of molecular weight of lignin during enzymatic polymerization was analyzed with gel permeation chromatography(GPC).Results indicated that the concentration of lignin in the effluent decreased from 93.1mg/L to 17.2mg/L after laccase treatment for 6h.Molecular weight of lignin isolated from the paper mill effluent was raised from 31,251 to 58,610 after 2h laccase treatment.In suitable conditions,the laccase produced by Coriolus versicolor could polymerize the lignin and its ramification in the wastewater of alkaline pulp mill to decrease CODCr and chroma in the wastewater,which could provide feasibility to reclaimed water reuse of the papermaking wastewater.
【Fund】: 国家自然科学基金项目资助(30471365 20674046)
【CateGory Index】: X793
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