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《Environmental Science & Technology》 2010-11
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Synergistic Efficiency of E.coli.Inactivation under US and UV

JIN Hui-xia1,DONG Bin1,SUN Ying2,LEI Zhao-wu2 (1.Department of Civil Construction,Ningbo Institute of Technology,Zhejiang University,Ningbo 315100,China;2.Department of Environment Engineering,Environmental Management College of China,Qinhuangdao 066004,China)  
Ultraviolet(UV),ultrasound(US)and synergistic efficiency were applied to inactivate E.coli,and the biological mechanism was investigated.Results showed that there was a good linear relation between UV irradiation dose and inactivation rate of E.coli.The power density of US had statistical significance on inactivation result.Operation condition of the maximum relative efficacy value was 7.18W/cm2 for 80s.The reaction rate constant increased from 0.1 to 0.1358 and photo reactivation rate of E.coli.decreased under the synergistic effect of US and UV.The cell structure of E.coli.was destroyed seriously under the synergistic effect of US and UV.US could not change DNA of E.coli.By specific recognition characteristics of T4 endonuclease V to thymine dimmer,DNA of E.coli.was found degradation under every operation condition and lots of CPDs were produced,and content of ESS produced by T4-Endo V digestion could embody inactivation rate of E.coli.under every operating condition.
【Fund】: 国家星火项目(2007EA701002);; 宁波市重大科技项目(2008C50018)
【CateGory Index】: R187
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