Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Environmental Science & Technology》 2012-12
Add to Favorite Get Latest Update

Study on a Denitrifier Strain:Its Identification,Functional Genes Detection and Denitrification Characteristics

HU Jin-xing1,2,TANG Jiang-wu2,WANG Xin2, YAO Xiao-hong2,WU Yi-fei2,GE Xiang-yang1(1.College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China; 2.Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Science,Hangzhou 310021,China)  
A high efficient strain of denitrifier ADH1 was isolated from activated sludge by enrichment culture and RTB plates,which was identified as Pseudomonas stutzeri through phylogenetic analysis based on its 16S rDNA sequence and biochemical characteristics,and in which two functional genes(nirS and nosZ)relating to denitrification were found implying the existence of a complete denitrifying enzyme system.The phylogenetic analysis also proved the strain of denitrifier ADH1 belonged to Pseudomonas sp.Findings of its denitrification showed that the conversion rate of nitrate went up 96.1% in 16 h under static cultivation and more than 84.7% on nitrate was totally removed from the cultural medium;on the other hand,under rocking cultivation condition(150 r/min)the conversion rate could reach 97.8% and a total nitrogen removal of 40% within 10 h.
【Fund】: 农业部“948”项目:基因网络和微纳米技术引进及在高效净水菌剂制备中的应用(2011-Z34)
【CateGory Index】: X172
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 7 Hits
1 CAI Long-yan,LI Ying,ZHENG Zi-hang(State Key Laboratory of Marine Environmental Science,Environmental Science Research Centre,Xiamen University,Fujian,Xiamen 361005,China);Temporal and Spatial Distribution of Nitrogen and Phosphorus of Lake Systems in China and Their Impact on Eutrophication[J];Earth and Environment;2010-02
2 YE Jiang-yu~(1,2),LUO Gu-yuan~(1,2),JI Fang-ying~(1,2),WANG Xi~(3),WANG Dan-yun~(2)(1.Key Laboratory of Eco-environments of Three Gorges Reservoir Region Under the State Ministry of Education;2.College of City Construction and Environmental Engineering,Chongqing University,Chongqing 400030,China3.Chongqing Academy of Environmental Science,Chongqing 400020,China);Functional Microbial Diversities in Wastewater Biological Treatment[J];Journal of Chongqing University(Natural Science Edition);2005-10
3 XIAO Jing-jing, GUO Ping, HUO Wei-jie, YU Jiang, ZHU Chang-xiong* (1.Institute of Agricultural Environment and Sustainable Development,CAAS,Beijing 100081,China; 2.Key Laboratory for Agro-environment and Climate Change,Ministry of Agriculture,Beijing 100081,China);Application of Denitrifying Microbes to Wastewater Denitrification[J];Environmental Science & Technology;2009-12
4 Fang Jing-jing,Ma Chuan-ming,Liu Cun-fu(School of Environmental Studies,China University of Geosciences,Wuhan 430074,China);The Advance of Study on Denitrifying Bacteria[J];Environmental Science & Technology;2010-S1
5 AN Yu-fa1,TAO Lei2,LIU Zhi-fei1(1.Department of Environmental and Municipal Engineering,Qingdao Technological University,Qingdao,Shandong 266033,China;2.Haibohe Sewerage-disposed Plant of Qingdao,Qingdao,Shandong 266021,China);Application of Biodenitrification in Pollution Control[J];Pollution Control Technology;2008-01
6 LIANG Li-Hua1,2 ZUO Jian-E1(1.Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China)(2.Department of Environmental Science,Northwest University,Xi'an,Shaanxi 710069,China);Denitrifying Functional Genes——the Molecular Marker for Detection of Denitrifying Community Structure[J];Microbiology;2009-04
7 WANG Wei1,CAI Zu-cong2,ZHONG Wen-hui3,WANG Guo-xiang4(1College of Life Science,Nanjing Normal University,Nanjing 210097,China;2State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China;3College of Chemistry and Environmental Science,Nanjing Normal University,Nanjing 210097,China;4College of Geographical Science,Nanjing Normal University,Nanjing 210097,China);Research advances in aerobic denitrifiers[J];Chinese Journal of Applied Ecology;2007-11
Chinese Journal Full-text Database 10 Hits
1 XIE Yong-mei,ZHANG Cheng-ling,ZHAO Yong-qiang,ZHANG Wei,ZHANG Guang-min*(Deptartment of Plant Protection,Shandong Agricultural University,Taian 271018,China);Identification and Biological Characteristics of the Pathogen of Black Rot on Chinese Cabbage in Shandong Province[J];Shandong Agricultural Sciences;2007-06
2 YAO Liang-tong1,DING Yan-qin1,LIU Yao1,CHEN Yi-ran1,DU Bing-hai1,LI Jun2(1.College of Life Sciences,Shandong Agricultural University,Taian 271018,China;2.Institute ofAgricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Screening and Identification of Bacteria Decomposing Maize Straw under Low Temperature and Their Effect Test[J];Shandong Agricultural Sciences;2008-03
3 ZHU Peng-ling1,2,CHEN Qiang1,DING Yan-qin2,YAO Liang-tong2,DU Zhi-bing2,WANG Jing2,DU Bing-hai2(1.College of Resources and Environmental Science,Sichuan Agricultural University,Yaan 625102,China; 2.College of Life Science,Shandong Agricultural University,Taian 271018,China);Screening and Identification of Antagonistic Bacteria against Rhizoctonia solani in Cotton Rhizosphere[J];Shandong Agricultural Sciences;2008-06
4 ZHANG Guang-zhi1,ZHOU Hong-zi1,YANG He-tong1,2,ZHAO Xiao-yan1(1.SINO-Japanese Friendship Biotechnology Research Center of Shandong Academy of Sciences,Shandong Provincial Key Lab for Applied Microbiology,Jinan 250014,China;2.Institute of Biology,Shandong Academy of Sciences,Jinan 250014,China);Isolation and Identification of Halotolerant Bacteria from Salt-soda Soil[J];Shandong Agricultural Sciences;2008-09
5 ZHANG Yi1,WANG Feng-zhong2,ZHANG Ming-shuo1,LI Yan1, HAN Ming-qu1,ZHOU Bo1,MAO Zhi-quan3(1.College of Life Science,Shandong Agricultural University/Shandong Provincial Key Laboratory of Microbiology,Taian 271018,China;2.Institute of Agro-products Processing Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China;3.College of Horticulture Science and Engineering,Shandong Agricultural University,Taian 271018,China);A Strain of Phlorhizin Degrading Bacterium Screened from Apple Orchard Soil[J];Shandong Agricultural Sciences;2011-12
6 LING Chen1,LIU Bo2(1.Economic & Technological Development Zone Branch of Hefei Environmental Protection Bureau,Hefei 230601,China;2.Anhui Research Institute of Chemical Industry,Hefei 230041,China);2,4,6-Tribromophenol Degrading Bacteria[J];Anhui Chemical Industry;2012-03
7 BAO Li-ning 1,2 ,HONG Gui-yun 1,HUANG Xian-huai 1,3 ( 1.Dept.of Environmental Engineering,Anhui Institute of Architecture & Industry, Hefei, 230022, China; 2.School of Science,Anhui Agriculture University, Hefei, 230036, China; 3.School of Materials Science and Chemistry, University of Science and Technology of China, Hefei, 230029, China );ANALYSIS OF DENITRIFYING BACTERIA IN BIOFILM-ELECTRODE PROCESS[J];Journal of Anhui Institute of Architecture;2004-05
8 Liuying1 et al.(1 College of Life Science,Shandong Normal University,Jinan 250014,China;2 College of Agriculture,Liaocheng University,Liaocheng 252059,China);Isolation,Identification and Drug Sensitivity of Bovine Mastitis Pathogenic Bacteria in west of Shandong[J];Anhui Agricultural Science Bulletin;2008-19
9 Cao Ning et al.;Isolation and Identification of Soil Salinization in Daqing[J];Anhui Agricultural Science Bulletin;2009-07
10 Zhao Jing et al.(School of Life Sciences,Fujian Agricultural and Forestry University,Fuzhou 350002,China);Isolation,Identification and Phylogenetie Analysis of the Cellulase-producing Strain[J];Anhui Agricultural Science Bulletin;2011-17
China Proceedings of conference Full-text Database 10 Hits
1 LIU Chun(Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou,510380,China);ISOLATED,IDENTIFICATION AND CHARACTERIZATION OF AEROMONAS SCHUBERTII FROM SNAKEHEAD[A];[C];2012
2 Huang Huawei~(1,2) Wang Yingeng~2 Chen Xia~3 Zhang Fengping~4 1 Fishery Sciences Institute of Hunan Province,Changsha,410153; 2 Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Qingdao,266071; 3 Sunny Oceans Aquaculture Technology Co.,Ltd,Qingdao 266071; 4 Fisheries Institute of Zhoushan,Zhoushan,316000;The Relation Between Skin Ulcer Syndrome Occurrence of Cultured Apostichopus Japonicus and Cultural Environment During Aestivation[A];[C];2011
3 YU Dong-You,LIU Shao-Na,LI Wei-Fen,Hang Hai-Zhi (Key Laboratory of Molecular Animal Nutrition of Chinese Ministry of Education Animal Science College, Zhejiang University;Hangzhou,310029);Isolation,Identification and characterization of a heterotrophic bacterium capable of removing nitrite[A];[C];2008
4 HU Zhi-gang~(1,2)HU Jiang-chun~(1**)LIU Li~1 WANG Si-long~1 SU Zhen-fei~3 WANG Shu-jin~1 (1 Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China;2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China;3 Yinling Forest Branch of Gaofeng Governmental Forestation,Nanning 530007,China);Composite preparation of marine microbes improves the soil quality in Eucalyptus plantation[A];[C];2008
5 Huang Xiaolan~(1,2),Chen Jianyao~1 1.School of Geographical Sciences and Planning,Zhongshan University,Guangzhou 510275,China; 2.SchooI of Resource and Environment Sciences,Guangxi Teachers Education University,Nanning 530001,China.;An overview on the Utilization Of Microorganism In Urban Wastewater And Sludge Treatment[A];[C];2008
6 Liu Aimin~1 Wang Min~1 Huang Weiyi~2 1 College of Life Sciences,Anhui Normal University,Wuhu,241000,China 2 College of Life Sciences,Nanjing Agriculture University,Nanjing,210095,China;Effects of antibiotics on the cadmium-tolerant capability of a Pseudomonas strain and IR spectroscopy analysis[A];[C];2008
7 LI Jun, SHU Wei-qun CHEN Ji'an, QIU Zhi-qun(Department of Environmental Hygiene, Third Military Medical University, Chongqing 400038,China);Study on isolation, identification and degradation characteristic of DBP-degradation strain CQ0302[A];[C];2005
8 XI Qing,ZHANG De-chun,GUAN Xiao-ran(Department of Pathogenic Biology,Molecular Medicine & Tumor Research Center,Chongqing Medical University,Chongqing 400016,China);Isolation and Preliminary Identification of Superior Acetobacter from Shanxi Grains vinegar[A];[C];2011
9 LI Mei,LIAO Baowen (The Research Institute of Tropical Forestry,CAF,Guangzhou 510520,China);Isolation and Screening of Hydrocarbon-degrading Bacterial Strains from the Mangrove Wetland Soils[A];[C];2011
10 Xiao Jingjing, Guo Ping, Tian Yunlong, Yu Jiang, Zhu Changxiong (Institute of Environment and Sustainable Development in Agriculture, CAAS, Beijing, 100081);Isolation and Identification of microbes from different humic acid materials[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Jiang-xia et al (School of Environmental Studies,China University of Geosciences,Wuhan,Hubei 430074);Process of Research on the Denitrification of Organism in Underground Water Using Solid Organic Carbon Sources and Its Influencing Factors[J];Journal of Anhui Agricultural Sciences;2008-04
2 WANG Bai-yang(Environmental Science and Engineering School,Nanchang University,Nanchang 330029,China);Denitrification Theory and Novel Processes of Biological Wastewater Treatment[J];Journal of Jiangxi Normal University(Natural Sciences Edition);2006-04
3 QUAN Xue-jun,XIAO Can,YANG Li-hua ,ZHANG Li-jie(College of Bioengineering, Chongqing Institute of Technology,Chongqing 400050, China);New Biological Denitrifying Technology for the Removal of NH3-N in Wastewater[J];Journal of Chongqing Institute of Technology;2005-08
4 LU Xi wu 1,LI Cong na 1,Yuhei Inamori 2 (1.Department of Environmental Engineering, Southeast University, Nanjing 210096,China; 2.National Institute for Environmental Studies, Tsukuba 305-0053,Japan);Effect of DO Concentration and MLSS on Simultaneous Nitrification and Denitrification[J];Urban Environment & Urban Ecology;2001-01
5 MA Jingan,LI Hongqing (Research Institute for Yangtze Water Resources Protection, Wuhan 430051,China);PRELIMINARY DISCUSSION ON EUTROPHICATION STATUS OF LAKES,RESERVOIRS AND RIVERS IN CHINA AND OVERSEAS[J];Resources and Environment in the Yangtze Basin;2002-06
6 Zhang Sheng 1,2,Li Chongming 1,Zhang Yong 1, Zhang Ke 1,Zheng Jian 1,Ye Cui 1 (1.Chongqing Institute of Environmental Science, Chongqing 400020; 2.South-West Agriculture University,Chongqing 400716);Eutrophication Investigation of Changshou Reservoir in Chongqing[J];Chongqing Environmental Science;2003-03
7 Zheng Hongai , Pan Lili, Huang Xiaohua,Yan Guoji (College of Biology and Environment Engineering, Zhejiang University of Technology, Hangzhou 310014);State and Controlling Measures on Xiazhu Lake's Eutrophication[J];Chongqing Environmental Science;2003-08
8 WANG Xiao-yu, FENG Jing, LI He(College of Urban and Environmental Sciences,Northeast Normal University, Changchun 130024, China);Research on variety of plankton community prior and post sediment dredging in Nanhu Lake,Changchun[J];Journal of Northeast Normal University (Natural Science Edition);2005-03
9 Wang Jun;Jiang Jianxiang;Lu Yuekwn;Wang Ning(Institute of Envtronmentol science, Northeast Normal University,Changchun,130024);Evaluation of the Lake and the Reservoir Nourishment in Jilin Province[J];JOURNAL OF NORTHEAST NORMAL UNIVERSITY (NATURAL SCIENCE EDITION);1994-02
10 CHEN Tongbin, ZENG Xibo, HU Qingxiu( Inst. of Geographic Sciences and Natural Resources Research, CAS, Beijing 100101, China; Inst. of Soil and Fertilizer, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Inst. of Agri-natural Resources &;Utilization Efficiency of Chemical Fertilizers among Different Counties of China[J];Acta Geographica Sinica;2002-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved