Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Environmental Science & Technology》 2012-12
Add to Favorite Get Latest Update

Interaction between Pb~(2+) and Multi-component Protein System

GUO Ming,HE Ling(Department of Chemistry,Zhejiang Agriculture and Forestry University,Lin'an 311300,China)  
Electrochemical method has been applied in studying mechanism of interaction between metal ions and protein molecule,in which electrodes are applied.In this paper,a chitosan-modified electrode was prepared through drop method and characterized by cyclic voltammetry and electrochemical impedance spectroscopy,which was used to probe into the mechanisms of interaction between Pb2+ and the systems of mixed protein and single component protein,suggesting a good linear response.The binding parameters of Pb2+ with protein were calculated by a theoretical equation and the experimental data indicated that in the mixed system existed the interaction between protein and protein molecules as well,thus affecting the interaction with single protein molecule.As a result,a calculation formula for measuring protein-protein interaction was proposed,which can be used to quantitatively measure the interaction of protein in a multi-component system.
【Fund】: 国家自然科学基金项目(20877072);; 浙江省科技创新项目资助(2010R412042)
【CateGory Index】: X171.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 Li Nanqiang Min Jing (Department of Chemistry,Peking University);POLAROGRAPHIC STUDIY ON THE NON-ELECTROACTIVE COMPLEX OF SCANDIUM-CARMINIC ACID[J];Chinese Journal of Analytical Chemistry;1989-04
2 Sun Wei, Jiao Kui *, Liu Xiaoyun (Department of Applied Chemistry, Qingdao Institute of Chemical Technology, Qingdao 266042);Electrochemical Study of Alizarin Red S-Bovine Serum Albumin Interaction[J];Chinese Journal of Analytieal Chemistry;2002-03
3 Ni Yongnian*, Du Shan(Department of Chemistry, Nanchang University, Nanchang 330047);Electrochemical and Spectral Investigation on Interaction Between Riboflavin with Deoxyribonucleic Acid[J];Chinese Journal of Analytical Chemistry;2006-05
4 ZHU Bing, DU Xiu Lian, LI Rong Chang, WANG Kui  (Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China) JIN Jian , WANG Bo Cheng ( National Key Laboratory of Nuclear Medicine, Wuxi 214063, Chin;Spectroscopic Studies on the Binding of Lanthanides to the Apolactoferrin[J];Chemical Research In Chinese Universities;2001-01
5 KANG Chun-Li,SU Chun-Yan,GUO Ping,ZHAO Yu-Xia,DONG De-Ming~*(College of Environment and Resources,Jilin University,Changchun 130021,China);Studies on Pb~(2+) and Cd~(2+) Adsorption by Extracellular Protein of Natural Biofilm[J];Chemical Journal of Chinese Universities;2006-07
6 CHEN Dan-dan~1,ZHANG Tao~1,REN Li-ping~(1*),SHI Deng-rong~2,YU Jin-lian~2 1.Department of Applied Chemistry,College of Science,China Agricultural University,Beijing 100094,China 2.Department of Environmental Science and Engineering,College of Tourism,Shanghai Normal University,Shanghai 200234,China;Study on the Spectra of Cal-Red-Cu(Ⅱ) Complex with Bovine Serum Albumin and Its Analytical Application[J];Spectroscopy and Spectral Analysis;2006-04
7 YU, Yong a,b HU, Jing Bo , a SHANG, Jun a LI, Qi Long a ( a Department of Chemistry, Beijing Normal University, Beijing 100875) ( b National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080);Study on the Electrochemical Behaviors of Vincristine and the Interaction of Vincristine with Tubulin[J];Acta Chimica Sinica;2004-02
8 Meng,Chenpeng Wang,Shun* Zhang,Kejun Jin,Huile* Chen,Xian Hu,Maolin Xiong,Jing(Nano-materials & Chemistry Key Laboratory,Wenzhou University,Wenzhou 325027);Electrochemical Study on the Interactions between Enantiomer of 2-(5-Fluorouracil-1-acetyl)amido-1,5-dimethyl Glutarate and Double-stranded/G-quadruplex DNA[J];Acta Chimica Sinica;2011-10
9 ZHANG Hai-Rong1 BIAN He-Dong*,1 NI Shou-Hai1 YU Qing 1 LIANG Hong*,2 CHEN Zhen-Feng2(1College of Chemistry and Chemical Engineering,Guangxi Normal University,Guilin,Guangxi 541004)(2Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources(MOE),Guangxi Normal University,Guilin,Guangxi 541004);Interaction of Pb(Ⅱ) with Bovine Serum Albumin under UV Irradiation[J];Chinese Journal of Inorganic Chemistry;2009-02
10 Lü Ya-Fen YIN Ya-Jing WU Ping CAI Chen-Xin (Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210097, P. R. China);Direct Electrochemistry and Bioelectrocatalysis of Myoglobin at a Carbon Nanotube-Modified Electrode[J];Acta Physico-Chimica Sinica;2007-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Nanqiang WANG Ronghui(Department of Chemistry,Peking University,Beijing,100871);Studies on the Voltammetric Behaviors of Rutin and Rare Earth Ions-Rutinsystem[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1994-04
2 WANG Ling1,ZHANG Zhan2,LIANG Rui-ling3(1.Zhengzhou Normal College,Zhengzhou 450044,China;2.Henan University of Construction,Pingdingshan 467036,China;3.Henan Center of Disease Control and Prevention,Zhengzhou 450016,China);Study on interaction of small molecules with protein[J];Journal of Henan University of Urban Construction;2010-05
3 JIANG Hong~(1,2) AN Pu-li~1 JIANG Ye~(1*) 1.Department of Analytical Chemistry,School of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China; 2.Equipment Center of Department of Training,Bethune Military Medical College,Shijiazhuang 050081;Drug and protein interactions:progress of research methods[J];Academic Journal of Second Military Medical University;2007-06
4 ZHANG Min CHENG Fa-Liang ZHANG Ming-Liang (Biosensor Research Center,Dongguan University of Technology,Dongguan 523808,China);Electrochemical Behaviors of Cytochrome C on Silver Nanowire Array Sensors[J];Journal of Dongguan University of Technology;2010-03
5 HE Xiao-min1,2,ZHANG Ting2,SHEN Min2,WEI Xing2,CHEN Hao2,HUANG Qiao-yun3(1.Hubei Environmental Monitoring Centre,Wuhan 430072,China;2.School of Science,Huazhong Agricultural University,Wuhan 430070,China;3.Key Laboratory of Subtropical Agricultural Resource and Environment of Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China);Adsorption of Pb~(2+) onto CTMAB-Montmorillonite-Pseudomonas putida Complex[J];Environmental Science & Technology;2010-07
6 CHEN Yi-ting,SU Bi-ling,HUANG Lu,LIN Qi (Chemistry and Chemical Engineering Department of Minjiang University,Fuzhou 350108,China);Determination of Dissociation Constant and Binding Constant of Famciclovir[J];Analysis and Testing Technology and Instruments;2011-01
7 Zhang Zhaohui, Zhang Yuexia( Central Iron & Sted Research Institute, MMI, Beijing 100081) Zhu Teng(Beijing University of Science and Technology, Beijing 100083);Complex Adsorptive Wave of Scandium (Ⅲ)-Chlorosulfophenol-S and Its Analytical Application[J];Chinese Journal of Analytical Chemistry;1999-04
8 Ma Weixing 1,2 , Qian Baohua 1,2 , Yang Xujie 1, Lu Lude 1 , Wang Xin 1, Li Ke′an 3 1(Materials Chemistry Laboratory, Nanjing University of Science and Technology, Nanjing 210094) 2(Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005) 3(College of Chemistry and Molecule Engineering, Peking University, Beijing 100871);Spectrophotometric Determination of Serum Proteins with Fast Green FCF[J];Chinese Journal of Analytical Chemistry;2003-12
9 Peng Zhen 1,2, Jiang Liping 1, Wang Ruixia 3, Song Feng 1, Lu Guanghan *1 1(College of Chemistry, Central China Normal University, Wuhan 430079) 2(Department of Chemistry, Chaohu University, Chaohu 238000) 3(Department of Chemistry, Chizhou Teacher′s College, Guichi 247000);Electrochemical Behavior of Alizarin Red S Interacting with Chitosan[J];Chinese Journal of Analytical Chemistry;2005-07
10 Sun Wei, Zhao Na, Xu Xiao, Jiao Kui(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042);Linear Sweep Polarographic Determination of Chondroitin Sulfate with Toluidine Blue as Electrochemical Probe[J];Chinese Journal of Analytical Chemistry;2007-11
China Proceedings of conference Full-text Database 2 Hits
1 ZHOU Wei-zhi~(1,2*),LI Wei-wei~1,ZHANG Yu-zhong~2 (1.School of Environmental Science and Engineering,Shandong University,Jinan 250100,China;2.State Key Lab of Microbial Technology,Shandong University,Jinan 250100,China);Pb~(2+) and Cu~(2+) Biosorption by Exopolysaccharide from the Deep-sea Psychrophilic Bacterium Pseudoalteromonas sp.SM9913[A];[C];2008
2 LU Kui~(*1,2) WANG Lan~1 MA Li~1 (1 School of Chemistry and Chemical Engineering,Henan University of Technology,Zhengzhou 450001; 2 Department of Material and Chemical Engineering,Henan Institute of Engineering,Zhengzhou 451191);Studies on the interaction of YAGFL with DNA[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Yanming (Department of Chemistry, Shanxi University)Wang Erkang (Changchun Institute of Applied Chemistry, Academia Sinica);VOLTAMMETRIC BEHAVIOUR OF BRILLIANT CRESYL BLUE[J];Chinese Journal of Analytical Chemistry;1985-02
2 Li Na; Li Kean; Tong Shenyang (Department of Chemistry, Peking University, Beijing 100871 );Spectrophotometric Study on the Interaction Between Arsenazo Ⅲ and Bovine Serum Albumin[J];Chinese Journal of Analytieal Chemistry;1996-01
3 Lin Xiuli(Shandong College of Traditional Chinese Medicine)Zhu Chenfu(Shandong University of Technology, Jinan 250014)Zou Shifu(Shandong University);Ultraviolet-Spectral properties of Copper(Ⅱ)-Amino Acid complexes and Determination of Their Composition and Stability Constants[J];Chinese Journal of Analytieal Chemistry;1996-02
4 Wei Yongju; Li Kean; Tong Shenyang(Department of Chemistry, Peking University, Beijing 100871);Interaction of Bromocresol Green With Serum Albumin in Acid Solution[J];Chinese Journal of Analytieal Chemistry;1996-04
5 Zhu Xiaofeng, Lin Bingcheng ( Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023);Measurement of Binding Constant of Host-Guest Interaction by Capillary Electrophoresis[J];Chinese Journal of Analytical Chemistry;1999-12
6 Yang Liju, Peng Tuzhi * (Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028);Development of Electrochemical Biosensor for Sequence-Specific Deoxyribonucleic Acid[J];Chinese Journal of Analytieal Chemistry;2001-03
7 Liu Huihong , Chen Xiantang, Li Jun (Department of Chemistry and Chemical Engineering, Xiangfan University, Xiangfan 441053) Hill H.A.O. (Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX13QR,UK);Direct Electrochemistry of Horseradish Peroxidase in Surfactant Films[J];Chinese Journal of Analytieal Chemistry;2001-05
8 Lu Xiaojun, Ju Huangxian * (Department of Chemistry, State Key Laboratory of Coordination Chemistry,Nanjing University, Nanjing 210093);Electrochemical Deoxyribonucleic Acid Biosensors and Their Applications[J];Chinese Journal of Analytical Chemistry;2003-01
9 Liu Yong~1,Xia Zhining~2,Li Hui~(1)~1(College of Chemical Engineering,Sichuan University,Chengdu 610065)~2(Institute of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400044);Capillary Electrophoresis Interaction Analysis for the Study of Binary Complex System[J];Chinese Journal of Analytical Chemistry;2005-12
10 Ni Yongnian*, Du Shan(Department of Chemistry, Nanchang University, Nanchang 330047);Electrochemical and Spectral Investigation on Interaction Between Riboflavin with Deoxyribonucleic Acid[J];Chinese Journal of Analytical Chemistry;2006-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved