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《Environmental Science & Technology》 2012-12
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Biodegradation of Chlorpyrifos by Manganese Peroxidase

MO Cai-ping1,ZHAO Lin-guo1,XIE Hui-fang2(1.School of Chemical Engineering,Nanjing Forestry University,Nanjing 210037,China; 2.School of Environmental and Biological Engineering,Nanjing University of Science and Technology,Nanjing 210094,China)  
Biodegradation of chlorpyrifos by manganese peroxidase(MnP) which was prepared using whit rot fungi P.sordida YK-624 was studied.The systems of chlorpytifos biodegradation were tested.Results showed that the chlorpyrifos biodegradation by MnP should be done in organic acid buffer solution(malonic acid)containing Mn2+ and H2O2 which was produced by glucose oxidase oxidizing glucose.But surfactant Tween 80 was not necessary because of poor solubility of chlorpyrifos.Main influencing factors were determined.It proved that chlorpyrifos could be biodegraded by MnP at 30 ℃ in pH 4.5 malonic acid buffer system,which contained 7.5 mmmol/L MnSO4,2.5 mmol/L glucose and 40 U glucose oxidase.Under the conditions,400 U/L MnP could degrade 77.51% 30 mg/L chlorpyrifos.GC-MS was used to analyze the product of chlorpyrifos biodegradation by MnP.It was found the possible enzymolysis product was O,O,O',O'-tetraethyl-dithiopyrophosphate,different from hydrolysis or bacterium degradation production of chlorpyrifos.
【Fund】: 引进国际先进林业科学技术计划项目(2011-4-15);; 南京林业大学科技创新基金(X09-031-1);; 江苏高校优势学科建设工程
【CateGory Index】: X172
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