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《Environmental Science & Technology》 2013-02
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Biomarkers of Serum Protein to Tetrabromobisphenol A in Carassius auratus

SUN Feng-chao 1,YANG Su-wen 2*,XU Fan-fan 2, DING Zhi-shan 1,ZHAO Ming-dong 2(1.Zhejiang Chinese Medical University,Hangzhou 310053,China; 2.Chinese Research Academy of Environmental Science,Beijing100012,China)  
Carassius auratus as test subject,acute toxicity of tetrabromobisphenol A(TBBPA),potential biomarkers of serum protein and toxicity target organ histopathology were studied.Results showed that 96 h-LC 50 of Carassius auratus exposed to TBBPA was 3.13 mg/L,and serum proteins of Carassius auratus can be divided into 11 components exposure to different concentrations TBBPA at different times.There were differences within protein components with time and concentration existed.Component 11 with clear bands and the largest amplitude first decreased then increased,and reached the highest value at low concentration on the 16th day,while it reached the highest value on the 2nd day at high concentration.The molecular weight of Component 11 was 24 kDa,may be the solution of polymer of calcitonin factor in coriouscles of stannius of Carassius auratus.The histopathological results indicated that Carassius auratus liver and kidney showed time-dose-dependent of pathological damage synchronously.The protein component 11 which responds sensitively on the concentration and time of TBBPPA inducing as well as easier to be recognized and identified can be used as the potential biomarkers in Carassius auratus exposed to TBBPA.
【Fund】: 国家重大科技专项水体污染控制与治理(2008ZX07105005)
【CateGory Index】: X174
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