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《Environmental Science & Technology》 2017-01
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A Broad-spectrum Keratin-degrading Strain:Screening and Degradation Characteristics

DU Dongxia;XU Jun;YIN Hongmei;LIU Biao;WANG Bin;HE Yuelin;Hunan Academy of Microbiology;  
A high-efficiency keratin-degrading strain(named E-26) was screened by enrichment cultivation and gradient acclimation.The strain was identified as Bacillus cereus on the basis of morphological examination,physiological and biochemical characterization,as well as 16 S rDNA sequence and phylogenetic analysis.The laboratory experiment results suggested,in terms of environmental factors and enzymology characteristics,that the best carbon and nitrogen source of E-26 degrading pig hairs were maltose and yeast powder;the optimum reaction condition of degrading pig hairs was:pH 8.0,temperature 40 ℃ inoculum dose 10%and substrate concentration 1%.Under the above mentioned optimized condition and fermental cultivation for 10 d,the keratinase activity of E-26 strain could reach 57.8 U/mL,37.4%higher than initial activity;and the rate of degradation of E-26 strain reached 71.2%,41.9%higher than the initial degradation rate.
【Fund】: 湖南省科技计划项目:无害化处理畜禽死体微生物菌剂研发(2015NK3057)
【CateGory Index】: X172
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1 HE Xipu;ZHOU Liwei;LIU Jie;LIU Hongjie;ZHANG Min;LI Pengfei;College of Chemistry and Chemical Engineering,Guangxi University;State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresource;College of Life Science and Technology,Guangxi University;Guangxi Key Laboratory of Chemistry and Engineering of Forest Products;;Isolation,Identification and Characteristics of a Feather-degrading Strain[J];环境科学与技术;2014-07
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 TAN Ying-ying, ZHENG Ping, HU Bao-lan, FENG Ji-qin(Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China);Feather degradation by Paecilomyces sp. isolated from sewage sludge[J];浙江大学学报(农业与生命科学版);2004-06
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