A PRELIMINARY STUDY ON THE CLADOSPORIN
Xie Qinmei Huang Jianhe ( Department of Forestry Science )
Cladosporium cladosporioides is the pathogen of moulds of shellac. Crude cladosporin was extracted from the mycelium of C. cladosporides by chloroform. The yellow oil substance pure cladosporin was obtained from crude cladosporin by sillica gel columu chromatography. The cladosporin can be dissolved in the organic solvent, and can not be dissolved in water. The Rf ( 0.56) was measured by thin layer chromatography. The 15% sucrose nutrient solution plus 2 % yeast extract and Czapek's nutrient solution plus 2% yeast extract most favored mycelia growth and cladosporin production. The ranges of optimum temperature and pH for growth were 25℃ and pH 6 - 7 respectively. Light and aeration had a some what effect on the mycelia growth and cladosporin productivity of the liquid culture. The growth peak of mycelia and the highest production of cladosporin were on the third week after inoculation. The cladosporin has a hard against effect on the Kerria lacca Kerr and Pan-onychus citri Mcgregor. The cladosporin exhipited the inhibitory effect on the Escherichia coli, Rhizoctonia solani, Trichoderma viride and Colletotrichum cam-eliae et al. at a concentration of 100-200μg/ml. Therefore, this cladosporin is a fine new microbial pesticide.