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《Journal of Fujian Forestry Science and Technology》 2008-01
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The optimization of ISSR-PCR conditions of Liquidambar formosana

BI Quan-xin1,JIN Ze-xin1,ZENG Zhi-cheng1,YE Wen-guo2,LI Jian-hui1 (1.Ecological Research Institute of Taizhou College,Linhai Zhejiang 317000,China;2.Tiantai County Forestry Bureau,Tiantai,Zhajiang 317200,China)  
The optimal conditions of ISSR amplification of Liquidambar formosana from Tiantai Mountain in Zhejiang Province was screened.After the analysis of the concentration Mg2+,4×dNTP,BSA,template DNA dosage,primer dosage,and unit of Taq DNA polymerase,the optimal conditions of ISSR-PCR was determined as follows: 1×Taq buffer(10 mmol·L-1 Tris-HCl,pH9.0,50 mmol·L-1 KCl and 0.1% Triton X-100),2.0 mmol·L-1 Mg2+,0.15 mol·L-1 4×dNTP,2.0 mg·mL-1 BSA,10 ng template DNA,6 pmol primers and 0.9 U Taq DNA polymerase;the suitable annealing temperature of UBC808 primer in the ISSR-PCR reaction system was 52.4℃.Using the optimal amplification,91 loci were produced in L.formosana population of Tiantai Mountain.The polymorphic loci percentage was 84.26%,Nei's gene diversity was 0.2665,and Shannon's information index was 0.4044.The genetic diversity of L.formosana was in a high level.
【Fund】: 浙江省自然科学基金资助项目(Y504220)
【CateGory Index】: S792.99
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Chinese Journal Full-text Database 1 Hits
1 WANG Wen-feng et al(College of Forestry Science,Gansu Agricultural University,Lanzhou,Gansu 730070);Establishment and Optimization of ISSR-PCR Reaction System for Picea crassifolia kom.[J];Journal of Anhui Agricultural Sciences;2009-21
Chinese Journal Full-text Database 5 Hits
1 CHEN Feng mao (General Station of Forest Disease and Insect Pest Control of Anhui Province,Hefei 230031,China);Analyses of Liquidambar formosana Seed Components[J];Journal of Fujian Forestry Science and Technology;2002-01
2 LI Jun-min, KE Shi-sheng, JIN Ze-xin ( Department of Biology and Chemistry, Taizhou Teachers` College, Linhai 317000, China );Extraction and determination of DNA from Heptacodium miconioides[J];Guihaia;2002-06
3 Fang LejinShi Jisen Li Li (College of Huangshan Huangshan 245041)(Nanjing Forestry University Nanjing 210037) Wu Xiaolong Shi Tingxian (Forestry Bureau of Qimen County, Anhui Province Qimen 245300);ANALYSIS OF GENETIC VARIATION OF PROGENY TRAITS IN LIQUIDAMBAR FORMOSANA[J];Scientia Silvae Sinicae;2003-03
4 BIAN Cai miao,LI Jun min,JIN Ze xin,GE Ming ju (Biology and Chemistry Department,Taizhou Teachers College,Linhai,Zhejiang 317000,China);Effect of BSA on Random Amplified Polymorphic DNA(RAPD) in Plants[J];Hereditas(Beijing);2002-03
5 JIN Ze-xin,LI Jun-min,LI Jian-hui(Institute of Ecology,Taizhou University,Linhai,Zhejing 317000,China);Genetic diversity of Schima superba populations revealed by ISSR analysis.[J];Journal of Zhejiang University(Agriculture and Life Sciences);2007-03
Chinese Journal Full-text Database 10 Hits
1 Zhang Deqiang;Zhang Zhiyi;Yang Kai.Institute of Populus tomentosa, Beijing For.Univ., 100083, P.R.China.\;;Applications of AFLP technique to genetic improvement in forest trees.[J];Journal of Beijing Forestry University;2000-06
2 JIN Ze-xin;LI Jun-min;LI Zeng-hong.Institute of Ecology,Taizhou University,Linhai City,Zhejiang Province,317000,P.R.China.;ISSR analysis of the genetic diversity of Torreya jackii Chun natural populations in Xianju County,Zhejiang Province.[J];Journal of Beijing Forestry University;2007-01
3 ZHANG Yu-rong~(1,2);LUO Ju-chun~1;YU Jin-xiu~2.1 College of Forestry,Beijing Forestry University,100083,P.R.China;2 Hunan Forestry Academy,Changsha,410004,P.R.China.;Genetic diversity of the endangered plant Abies ziyuanensis detected by ISSR markers.[J];Journal of Beijing Forestry University;2007-06
4 Chen Zuyun Wang Xiaoli Song Juxian(Guiyang Medical University,Guiyang 550004);Genetic Polymorphism of Gastrodia elata Bl.Populations from Guizhou Based on ISSR Analysis[J];China Journal of Traditional Chinese Medicine and Pharmacy;2007-07
5 ZHANG Li rong, XU Da qing, LIU Da qun (College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China);SSR marker, ISSR marker and their application to plant genetics and breeding[J];Journal of Agricultural University of Hebei;2002-01
6 Ren Xuqin;. WangQiuyu(Northeast Forestry University, Harbin 150040. P. R. China); Liu Huaibing(Multi- management Bureau of Huaiyin City);The Optimal Phase for Extracting DNA from Endosperms of Picea koraiensis Nakai[J];Journal of Northeast Forestry University;2001-03
7 Hu Shourong(Garden Department in Harbin, Harbin 150080. P. R. China); Xia Ming(Zhejiang University); Guo Changying(Garden Department in Harbin): Lu Xiaochun(Zhejiang University);Methods of Genetic Diversity for Forest-tree[J];Journal of Northeast Forestry University;2001-03
8 Liu Ming, Wang Jihua, Wang Tongchang(Har bin Normal University, Harbin 150080, P. R. China);DNA Molecular Markers[J];Journal of Northeast Forestry University;2003-06
9 ZHANG Hong-yi,CHEN Yue-qin,LIAO Wen-bo~*(School of Life Sciences,Zhongshan University,Guangzhou 510275,China);RAPD analysis of population genetic diversity of Taxus mairei[J];Acta Botanica Boreali-occidentalia Sinica;2003-11
10 TANG Yu-hai~1,GUO Chun-fang~(1,2),ZHANG Mu-qing~1,CHEN Chang-song~3,CHEN Rong-bing~3(1.Key Laboratory of Eco-physiology and Genetic Improvement for Sugarcane,Ministry of Agriculture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;2.Fujian Institute of Education,Fuzhou,Fujian 350025,China;3.Tea Research Institute,Fujian Academy of Agriculture Sciences,Fu′an,Fujian 355015,China);Application of ISSR markers to the genetic polymorphism of Camellia sinensis[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2007-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LI Jun-min, KE Shi-sheng, JIN Ze-xin ( Department of Biology and Chemistry, Taizhou Teachers` College, Linhai 317000, China );Extraction and determination of DNA from Heptacodium miconioides[J];Guihaia;2002-06
2 DING Sheng_Yan(College of Environment & Planning,Hennan University,Kaifeng 475001, Henan, China);The Causes of Castanopsis Fargesii and Schima Supe rba Being Dominant Species of Series of Evergreen Broad-Leaved Forest in Zhejiang Tiantong[J];Journal of Henan University (Natural Science);2001-01
3 Chen Youdi (Research Institute of Chemical Processing and Utilization of Forest Products, Chinese Academy of Forestry Nanjing 210037) Hu Zhidong Gu Yin (Jiangsu Institute of Botany, Nanjing 210014);STUDY ON CHEMICAL COMPOSITION OF FLAVONOIDS AND TERPINENES IN LIQUIDAMBAR[J];Chemistry & Industry of Forest Products;1991-02
4 Zhang Ping1 Zhou Zhichun1 Jin Guoqing1 Fan Huihua2 Hu Hongbao3(1. The Research Institute of Subtropical Forestry, CAF Fuyang 311400; 2. The Extending Center for Forestry Science and Technology of Jian'ou city, Fujian Province Jian'ou 353100; 3. Forestry Bureau of Chun'an County, Zhejiang Province Chun'an 311700);Genetic Diversity Analysis and Provenance Zone Allocation of Schima superba in China Using RAPD Markers[J];Scientia Silvae Sinicae;2006-02
5 Gao Handong\ Chen Fengmao\ Shi Jisen(College of Forest Resources and Environment Nanjing Forestry University\ Nanjing\ 210037);A Study on Maturation of Liquidambar formosana Seeds[J];JOURNAL OF NANJING FORESTRY UNVERSITY;2000-03
6 Yin TongmingHan ZhengminHuang MinrenWang MingxiuLi Shuxian (Forest Genetics and Gene Engineering Laboratory Nanjing Forestry UniversityNanjing210037);RAPD Experiment Protocol and Optimization in Forest Trees[J];JOURNAL OF NANJING FORESTRY UNVERSITY;1999-04
7 YE Bing-ying 1,CHEN You-qiang 1,ZHU Jin-mao 1,ZHUANG Wei-jian 2 (1. Bio-engineering College,Fujian Teachers' University,Fuzhou 350007,China; 2. Crop College, Fujian Agriculture University);Study on RAPD Reaction Conditions for Peanut (Arachis hypogaea L.)[J];PEANUT SCIENCE AND TECHNOLOGY;2000-01
8 Liu Kangde; Li Jianguo; Peng Shiqin; Zheng Xueqin(Tropical Horticulture Institute, CATAS, Danzhou, Hainan, 571737; National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou, Hainan, 571101);Genome DNA Extraction and RAPD Analysis of Avocado[J];CHINESE JOURNAL OF TROPICAL CROPS;1999-04
9 LI Ang, GE Song Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093;Advances in plant conservation genetics[J];Chinese Biodiversity;2002-01
10 ZUO Kai-Jing, SUN Ji-Zhong , ZHANG Jin-Fa, NIE Yi-Chun, LIU Jin-Lan (National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China);Genetic Diversity Evaluation of Some Chinese Elite Cotton Varieties with RAPD Markers[J];ACTA GENETICA SINICA;2000-09
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