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《Journal of Fujian Forestry Science and Technology》 2018-02
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Screening of Proliferation and Rooting Medium for Tissue Culture of Toona sinensis

GAO Yanming;XIAO Xiangxi;WANG Zhijie;ZHANG Haiyan;HE Wenguang;GAO Nan;XIAO Huashan;Fujian Academy of Forestry;College of Life Science,Fujian Normal University;Forestry Science and Technology Extension Center of Shaowu City;  
In order to study the tissue culture and rapid propagation of Toona sinensis,the medium formula for tissue culture of Toona sinensis was studied and screened.By adding different concentrations of 6-BA,NAA and GA3 in MS medium,the orthogonal experiment and variance analysis showed that the main factor affecting the proliferation of Toona sinensis was 6-BA,which had a significant effect on the bud proliferation(P 0.05),and the best concentration was 1.0 mg·L~(-1);the secondary factor affecting bud proliferation was NAA,and the optimal concentration was 0.1 mg·L~(-1);GA3 had the least effect on bud proliferation,but GA3 had a promoting effect on the internode elongation growth of young stems.The optimal bud culture medium combination was MS + 6-BA 1.0 mg·L~(-1)+ NAA 0.1 mg·L~(-1)+ GA30.1 mg·L~(-1),and the multiplication ratio of shoots reached 3.6 times.MS and 1/2 MS medium supplemented with different concentrations of NAA and IBA to carry out rooting culture test,the results showed that the MS + NAA0.2 mg·L~(-1)+ IBA 0.2 mg·L~(-1) formula combination medium had the best rooting effect,and the rooting rate was 25%.Taking humus soil as tissue culture seedlings transplanting toothache,the survival rate of the tissue culture seedlings transplanted was high up to 95%,and the seedlings grew well.
【Fund】: 福建省属公益类科研院所基本科研专项(2017R1011-1);; 福建省第四期林木种苗科技攻关项目(闽林科[2013]1号);; 福州市科技计划项目(榕科[2014]103号)
【CateGory Index】: S792.99
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