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《Journal of Fujian Forestry Science and Technology》 2019-02
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Extraction and Analysis of Genomic DNA from Leaves of Castanopsis carlesii

LIN Lili;GUO Liqian;CHEN Xiaoxiao;YOU Zhangtian;YOU Shuisheng;CAO Shijiang;College of Forestry,Fujian Agriculture and Forestry University;College of Agriculture & Biotechenology,Zhejiang University;Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems in South China Botanical Garden,Chinese Academy of Sciences,Key Laboratory of Applied Botany of Guangdong Province,South China Botanical Garden,Chinese Academy of Sciences;  
In this study,taking the leaves of carlesii community of Castanopsis carlesii as materials,the modified CTAB method was adopted to extract the genome DNA of Castanopsis carlesii,and the obtained genomic DNA was tested for concentration,PCR amplification,agarose gel electrophoresis detection,content determination analysis and amplification result sequencing.The results showed that the quality and concentration of genomic DNA extracted from Castanopsis carlesii leaves were good,with good integrity,good PCR effect,clear display of electrophoretic mapping strips,and the length of sequencing results met the requirements.The DNA extracted by this method can be used for the analysis of genetic diversity of Castanopsis carlesii,which will provide reference for the DNA extraction and other molecular research of other Castanopsis plants,such as Castanopsis carlesii and Castanopsis kawakamii.
【Fund】: 福建省林业推广项目(K8517202A);; 福建农林大学A类毕业生科研启动费(KXMLT001A)
【CateGory Index】: S792.17
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