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Establishment of cDNA-AFLP Analysis System with Two Low-frequency Restriction Enzyme in Acacia melanoxylon

HU Wei(College of Life Sciences,Fujian Normal University,Fuzhou 350117,China)  
An optimized system was established after studying on the factors affecting cDNA-AFLP analysis system of Acacia melanoxylon cultured in vitro.The results indicated that the restriction enzyme combination was EcoRⅠ and PstⅠ,the dosage of dscDNA was 500 ng,the ligation products using as template of pre-amplification were diluted to one time,and the pre-amplification products using as template of select-amplification were diluted to 50 times.
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